Guidelines for the Isolation and Characterization of Murine Vascular Smooth Muscle Cells. A Report from the International Society of Cardiovascular Translational Research

Neeta Adhikari; Kadambari Chandra Shekar; Rodney Staggs; Zaw Win; Kerianne Steucke; Yi Wei Lin; Li Na Wei; Patrick Alford; Jennifer L. Hall

doi:10.1007/s12265-015-9616-6

Guidelines for the Isolation and Characterization of Murine Vascular Smooth Muscle Cells. A Report from the International Society of Cardiovascular Translational Research

Neeta Adhikari, Kadambari Chandra Shekar, Rodney Staggs, Zaw Win, Kerianne Steucke, Yi Wei Lin, Li Na Wei, Patrick Alford, Jennifer L. Hall

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Vascular smooth muscle cells (VSMCs) play important roles in cardiovascular disorders and biology. Outlined in this paper is a step-by-step procedure for isolating aortic VSMCs from adult C57BL6J male mice by enzymatic digestion of the aorta using collagenase. The plating, culturing, and subculturing of the isolated cells are discussed in detail along with techniques to characterize VSMC phenotype by gene expression and immunofluorescence. Traction force microscopy was used to characterize contractility of single subcultured VSMCs at baseline.

Original language	English (US)
Pages (from-to)	158-163
Number of pages	6
Journal	Journal of cardiovascular translational research
Volume	8
Issue number	3
DOIs	https://doi.org/10.1007/s12265-015-9616-6
State	Published - Apr 1 2015

Bibliographical note

Publisher Copyright:
© 2015, Springer Science+Business Media New York.

Keywords

Aorta
Caldesmon1
Calponin 1
Murine
Smooth muscle 22α
Smooth muscle myosin heavy chain
Smooth muscle α-actin
Smoothelin
Subculture
Vascular smooth muscle cell

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Adhikari, N., Shekar, K. C., Staggs, R., Win, Z., Steucke, K., Lin, Y. W., Wei, L. N., Alford, P., & Hall, J. L. (2015). Guidelines for the Isolation and Characterization of Murine Vascular Smooth Muscle Cells. A Report from the International Society of Cardiovascular Translational Research. Journal of cardiovascular translational research, 8(3), 158-163. https://doi.org/10.1007/s12265-015-9616-6

Guidelines for the Isolation and Characterization of Murine Vascular Smooth Muscle Cells. A Report from the International Society of Cardiovascular Translational Research. / Adhikari, Neeta; Shekar, Kadambari Chandra; Staggs, Rodney et al.
In: Journal of cardiovascular translational research, Vol. 8, No. 3, 01.04.2015, p. 158-163.

Research output: Contribution to journal › Article › peer-review

Adhikari, N, Shekar, KC, Staggs, R, Win, Z, Steucke, K, Lin, YW, Wei, LN , Alford, P & Hall, JL 2015, 'Guidelines for the Isolation and Characterization of Murine Vascular Smooth Muscle Cells. A Report from the International Society of Cardiovascular Translational Research', Journal of cardiovascular translational research, vol. 8, no. 3, pp. 158-163. https://doi.org/10.1007/s12265-015-9616-6

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Guidelines for the Isolation and Characterization of Murine Vascular Smooth Muscle Cells. A Report from the International Society of Cardiovascular Translational Research

Abstract

Bibliographical note

Keywords

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