Guidelines for the Isolation and Characterization of Murine Vascular Smooth Muscle Cells. A Report from the International Society of Cardiovascular Translational Research

Neeta Adhikari, Kadambari Chandra Shekar, Rodney Staggs, Zaw Win, Kerianne Steucke, Yi Wei Lin, Li Na Wei, Patrick Alford, Jennifer L. Hall

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Vascular smooth muscle cells (VSMCs) play important roles in cardiovascular disorders and biology. Outlined in this paper is a step-by-step procedure for isolating aortic VSMCs from adult C57BL6J male mice by enzymatic digestion of the aorta using collagenase. The plating, culturing, and subculturing of the isolated cells are discussed in detail along with techniques to characterize VSMC phenotype by gene expression and immunofluorescence. Traction force microscopy was used to characterize contractility of single subcultured VSMCs at baseline.

Original languageEnglish (US)
Pages (from-to)158-163
Number of pages6
JournalJournal of cardiovascular translational research
Volume8
Issue number3
DOIs
StatePublished - Apr 1 2015

Keywords

  • Aorta
  • Caldesmon1
  • Calponin 1
  • Murine
  • Smooth muscle 22α
  • Smooth muscle myosin heavy chain
  • Smooth muscle α-actin
  • Smoothelin
  • Subculture
  • Vascular smooth muscle cell

Fingerprint

Dive into the research topics of 'Guidelines for the Isolation and Characterization of Murine Vascular Smooth Muscle Cells. A Report from the International Society of Cardiovascular Translational Research'. Together they form a unique fingerprint.

Cite this