H9c2 cell line is a valuable in vitro model to study the drug metabolizing enzymes in the heart

Beshay N.M. Zordoky, Ayman O.S. El-Kadi

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

Introduction: Recent studies demonstrated that cultured primary cardiomyocytes are a valuable tool for studying the metabolic capacity of the heart. However, a major limitation for isolated cardiomyocytes is that they are rather fragile and difficult to isolate. Therefore, there is an urgent need for an in vitro cell line model. Methods: Expression of different cytochrome P450 (CYP) genes were examined in a rat H9c2 cell line in comparison with that of rat heart. RNAs from H9c2 cells, rat heart as well as rat liver were isolated and CYP mRNA expression was determined by reverse transcription-polymerase chain reaction. Results: Our results showed that CYP1A1 and 1B1 are constitutively expressed in both H9c2 cells and the heart. CYP1A1 was induced by β-naphthoflavone in H9c2 cells and the heart, whereas CYP1B1 was only induced in the heart. CYP2B1, 2B2, 2E1 and 2J3 were expressed in H9c2 cells and the heart at a comparable level but significantly lower than that detected in the liver. Expression of CYP2C11, 2C13, and 2C23 appeared to be greater in the cell line than in heart. On the other hand, CYP2A1, 3A1, and 3A2 were not expressed either in H9c2 cells or in the heart. Discussion: Our findings provide the first evidence for the expression of multiple CYPs in H9c2 cells at comparable levels to those expressed in the rat heart. Therefore, this cell line offers a valuable in vitro model to study the metabolic capacity of the heart.

Original languageEnglish (US)
Pages (from-to)317-322
Number of pages6
JournalJournal of Pharmacological and Toxicological Methods
Volume56
Issue number3
DOIs
StatePublished - Nov 2007
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by a grant from the Heart and Stroke Foundation of Alberta, NWT, and Nunavut to A.O.S.E. B.N.M.Z. is the recipient of Egyptian Government Scholarship.

Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.

Keywords

  • Cardiomyocytes
  • Cytochrome P450s
  • Drug metabolizing enzymes
  • H9c2 cells
  • Heart

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