Haloacetonitriles Are Low KI Inhibitors of Bacterial Dichloromethane Dehalogenases

Michael S.P. Logan, Frank A. Blocki, Kurt J. Stimpfl, Lawrence P. Wackett

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Distinct dichloromethane dehalogenases from Methylobacterium sp. strain DM4 and Methylophilus DM11 were inhibited by low concentrations of haloacetonitriles. Chloroacetonitrile (ClCH2CN) showed maximal inhibition at a stoichiometry of 1 mol inhibitor: 1 mol holoenzyme for both enzymes. This stoichiometry is suggestive of one active site per holoenzyme or extreme negative cooperativity amongst the subunits. Radiolahcllcd ClCH2CN dissociated completely or partially from the two dehalogenases, respectively, during chromatography. This suggested ClCH2CN was bound non-covalently.

Original languageEnglish (US)
Pages (from-to)853-858
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume197
Issue number2
DOIs
StatePublished - 1993

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