Hepatic D‐galactosamine toxicity studied with localized in Vivo 31P magnetic resonance spectroscopy in intact rats

Sally Weisdorf, Kristy Hendrich, Steven Buchthal, John Wike, Gerald Bratt, Hellmut Merkle, Michael Garwood, Kamil Ugurbil

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Spatially resolved 13P magnetic resonance spectroscopy (MRS) at 4.7 T was applied to noninvasively assess liver phosphorus metabolites in a biochemically well‐characterized model of hepatotoxicity induced by injection of a sublethal dose of D‐galactosamine (galN). A newly developed hybrid method based on spectral localization with B0 and B1 gradients was employed to obtain multivoxel spectra in intact anesthesized rats. Spatially localized in vivo spectra were recorded 0 to 26 h after galN injection of female rats. In response to galN exposure, diphosphodiester peaks ascribed to UDP‐hexosamines became detectable by 4 h and persisted up to 26 h. A metabolite coresonating with inorganic phosphate increased rapidly in intensity by 2 h after galN and returned to baseline by 18 h; this resonance was shown not to be Pi and was assigned to galN‐1‐phosphate by subsequent high resolution MRS experiments on extracts prepared from these livers. These results confirmed in vivo the metabolic perturbations described previously for this model of hepatotoxicity following biochemical studies based on classical extraction methods. Unlike the in vitro studies, however, these noninvasive experiments provided additional information on the time course of metabolic alterations on the same animal. © 1991 Academic Press, Inc.

Original languageEnglish (US)
Pages (from-to)178-190
Number of pages13
JournalMagnetic resonance in medicine
Volume21
Issue number2
DOIs
StatePublished - Oct 1991

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