TY - JOUR
T1 - Hepatic D‐galactosamine toxicity studied with localized in Vivo 31P magnetic resonance spectroscopy in intact rats
AU - Weisdorf, Sally
AU - Hendrich, Kristy
AU - Buchthal, Steven
AU - Wike, John
AU - Bratt, Gerald
AU - Merkle, Hellmut
AU - Garwood, Michael
AU - Ugurbil, Kamil
PY - 1991/10
Y1 - 1991/10
N2 - Spatially resolved 13P magnetic resonance spectroscopy (MRS) at 4.7 T was applied to noninvasively assess liver phosphorus metabolites in a biochemically well‐characterized model of hepatotoxicity induced by injection of a sublethal dose of D‐galactosamine (galN). A newly developed hybrid method based on spectral localization with B0 and B1 gradients was employed to obtain multivoxel spectra in intact anesthesized rats. Spatially localized in vivo spectra were recorded 0 to 26 h after galN injection of female rats. In response to galN exposure, diphosphodiester peaks ascribed to UDP‐hexosamines became detectable by 4 h and persisted up to 26 h. A metabolite coresonating with inorganic phosphate increased rapidly in intensity by 2 h after galN and returned to baseline by 18 h; this resonance was shown not to be Pi and was assigned to galN‐1‐phosphate by subsequent high resolution MRS experiments on extracts prepared from these livers. These results confirmed in vivo the metabolic perturbations described previously for this model of hepatotoxicity following biochemical studies based on classical extraction methods. Unlike the in vitro studies, however, these noninvasive experiments provided additional information on the time course of metabolic alterations on the same animal. © 1991 Academic Press, Inc.
AB - Spatially resolved 13P magnetic resonance spectroscopy (MRS) at 4.7 T was applied to noninvasively assess liver phosphorus metabolites in a biochemically well‐characterized model of hepatotoxicity induced by injection of a sublethal dose of D‐galactosamine (galN). A newly developed hybrid method based on spectral localization with B0 and B1 gradients was employed to obtain multivoxel spectra in intact anesthesized rats. Spatially localized in vivo spectra were recorded 0 to 26 h after galN injection of female rats. In response to galN exposure, diphosphodiester peaks ascribed to UDP‐hexosamines became detectable by 4 h and persisted up to 26 h. A metabolite coresonating with inorganic phosphate increased rapidly in intensity by 2 h after galN and returned to baseline by 18 h; this resonance was shown not to be Pi and was assigned to galN‐1‐phosphate by subsequent high resolution MRS experiments on extracts prepared from these livers. These results confirmed in vivo the metabolic perturbations described previously for this model of hepatotoxicity following biochemical studies based on classical extraction methods. Unlike the in vitro studies, however, these noninvasive experiments provided additional information on the time course of metabolic alterations on the same animal. © 1991 Academic Press, Inc.
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U2 - 10.1002/mrm.1910210203
DO - 10.1002/mrm.1910210203
M3 - Article
C2 - 1660559
AN - SCOPUS:0025934550
SN - 0740-3194
VL - 21
SP - 178
EP - 190
JO - Magnetic resonance in medicine
JF - Magnetic resonance in medicine
IS - 2
ER -