Heterotrophic cultivation of Chlorella vulgaris using broken rice hydrolysate as carbon source for biomass and pigment production

Yihui Cai; Yuhuan Liu; Tongying Liu; Kaili Gao; Qi Zhang; Leipeng Cao; Yunpu Wang; Xiaodan Wu; Hongli Zheng; Hong Peng; Roger Ruan

doi:10.1016/j.biortech.2020.124607

Heterotrophic cultivation of Chlorella vulgaris using broken rice hydrolysate as carbon source for biomass and pigment production

Yihui Cai, Yuhuan Liu, Tongying Liu, Kaili Gao, Qi Zhang, Leipeng Cao, Yunpu Wang, Xiaodan Wu, Hongli Zheng, Hong Peng, Roger Ruan

Bioproducts and Biosystems Engineering

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15 Scopus citations

Abstract

The high cost of carbon source limits the heterotrophic culture of Chlorella. In this study, broken rice was hydrolyzed into glucose. Then, the broken rice hydrolysate (BRH) was utilized for heterotrophic cultivation of C. vulgaris instead of glucose. Results showed that algal cells released H⁺ when they consumed NH₄⁺, leading to a sharp decrease in pH. Growth inhibition by acid could be avoided by using a pH buffer. Adding alkaline reagents intermittently during culture could not only reduce the amount of pH stabilizer but also obtain increased biomass production. When using Tris as pH stabilizer, the biomass productivity of C. vulgaris in BRH was the largest (1.01 g/L/d), followed by NaOH (1.00 g/L/d), and Na₂CO₃ (0.95 g/L/d). Using BRH instead of glucose for heterotrophic cultivation of C. vulgaris could save 89.58% of the cost of culture medium. This study developed a novel strategy for cultivating C. vulgaris heterotrophically using BRH.

Original language	English (US)
Article number	124607
Journal	Bioresource Technology
Volume	323
DOIs	https://doi.org/10.1016/j.biortech.2020.124607
State	Published - Mar 2021

Bibliographical note

Funding Information:
This research was supported by the National Natural Science Foundation of China (21878237, 21878139, 51708308, 21767017 and 22066016) in China; the Key Research and Development Program of Jiangxi Province (20181BAB206030, 2020BAB203012 and 20181BBF60026), the research project of State Key Laboratory of Food Science and Technology, Nanchang University (Project No. SKLF-ZZB-201915 and 201722) in China; the Post-doctoral Innovative Talents Support Program (BX20190147); and the selected funding for post-doctoral research projects in Jiangxi Province (2019KY04).

Publisher Copyright:
© 2020 Elsevier Ltd

Keywords

Broken rice hydrolysate
Carbon source
Chlorella vulgaris
Heterotrophic culture
Pigment

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title = "Heterotrophic cultivation of Chlorella vulgaris using broken rice hydrolysate as carbon source for biomass and pigment production",

abstract = "The high cost of carbon source limits the heterotrophic culture of Chlorella. In this study, broken rice was hydrolyzed into glucose. Then, the broken rice hydrolysate (BRH) was utilized for heterotrophic cultivation of C. vulgaris instead of glucose. Results showed that algal cells released H+ when they consumed NH4+, leading to a sharp decrease in pH. Growth inhibition by acid could be avoided by using a pH buffer. Adding alkaline reagents intermittently during culture could not only reduce the amount of pH stabilizer but also obtain increased biomass production. When using Tris as pH stabilizer, the biomass productivity of C. vulgaris in BRH was the largest (1.01 g/L/d), followed by NaOH (1.00 g/L/d), and Na2CO3 (0.95 g/L/d). Using BRH instead of glucose for heterotrophic cultivation of C. vulgaris could save 89.58% of the cost of culture medium. This study developed a novel strategy for cultivating C. vulgaris heterotrophically using BRH.",

keywords = "Broken rice hydrolysate, Carbon source, Chlorella vulgaris, Heterotrophic culture, Pigment",

author = "Yihui Cai and Yuhuan Liu and Tongying Liu and Kaili Gao and Qi Zhang and Leipeng Cao and Yunpu Wang and Xiaodan Wu and Hongli Zheng and Hong Peng and Roger Ruan",

note = "Funding Information: This research was supported by the National Natural Science Foundation of China (21878237, 21878139, 51708308, 21767017 and 22066016) in China; the Key Research and Development Program of Jiangxi Province (20181BAB206030, 2020BAB203012 and 20181BBF60026), the research project of State Key Laboratory of Food Science and Technology, Nanchang University (Project No. SKLF-ZZB-201915 and 201722) in China; the Post-doctoral Innovative Talents Support Program (BX20190147); and the selected funding for post-doctoral research projects in Jiangxi Province (2019KY04). Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",

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T1 - Heterotrophic cultivation of Chlorella vulgaris using broken rice hydrolysate as carbon source for biomass and pigment production

AU - Cai, Yihui

AU - Liu, Yuhuan

AU - Liu, Tongying

AU - Gao, Kaili

AU - Zhang, Qi

AU - Cao, Leipeng

AU - Wang, Yunpu

AU - Wu, Xiaodan

AU - Zheng, Hongli

AU - Peng, Hong

AU - Ruan, Roger

N1 - Funding Information: This research was supported by the National Natural Science Foundation of China (21878237, 21878139, 51708308, 21767017 and 22066016) in China; the Key Research and Development Program of Jiangxi Province (20181BAB206030, 2020BAB203012 and 20181BBF60026), the research project of State Key Laboratory of Food Science and Technology, Nanchang University (Project No. SKLF-ZZB-201915 and 201722) in China; the Post-doctoral Innovative Talents Support Program (BX20190147); and the selected funding for post-doctoral research projects in Jiangxi Province (2019KY04). Publisher Copyright: © 2020 Elsevier Ltd

PY - 2021/3

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N2 - The high cost of carbon source limits the heterotrophic culture of Chlorella. In this study, broken rice was hydrolyzed into glucose. Then, the broken rice hydrolysate (BRH) was utilized for heterotrophic cultivation of C. vulgaris instead of glucose. Results showed that algal cells released H+ when they consumed NH4+, leading to a sharp decrease in pH. Growth inhibition by acid could be avoided by using a pH buffer. Adding alkaline reagents intermittently during culture could not only reduce the amount of pH stabilizer but also obtain increased biomass production. When using Tris as pH stabilizer, the biomass productivity of C. vulgaris in BRH was the largest (1.01 g/L/d), followed by NaOH (1.00 g/L/d), and Na2CO3 (0.95 g/L/d). Using BRH instead of glucose for heterotrophic cultivation of C. vulgaris could save 89.58% of the cost of culture medium. This study developed a novel strategy for cultivating C. vulgaris heterotrophically using BRH.

AB - The high cost of carbon source limits the heterotrophic culture of Chlorella. In this study, broken rice was hydrolyzed into glucose. Then, the broken rice hydrolysate (BRH) was utilized for heterotrophic cultivation of C. vulgaris instead of glucose. Results showed that algal cells released H+ when they consumed NH4+, leading to a sharp decrease in pH. Growth inhibition by acid could be avoided by using a pH buffer. Adding alkaline reagents intermittently during culture could not only reduce the amount of pH stabilizer but also obtain increased biomass production. When using Tris as pH stabilizer, the biomass productivity of C. vulgaris in BRH was the largest (1.01 g/L/d), followed by NaOH (1.00 g/L/d), and Na2CO3 (0.95 g/L/d). Using BRH instead of glucose for heterotrophic cultivation of C. vulgaris could save 89.58% of the cost of culture medium. This study developed a novel strategy for cultivating C. vulgaris heterotrophically using BRH.

KW - Broken rice hydrolysate

KW - Carbon source

KW - Chlorella vulgaris

KW - Heterotrophic culture

KW - Pigment

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Heterotrophic cultivation of Chlorella vulgaris using broken rice hydrolysate as carbon source for biomass and pigment production

Abstract

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