High-efficiency filter fluorometer for capillary electrophoresis and its application to fluorescein thiocarbamyl amino acids

Edgar Arriaga, Da Yong Chen, Xiao Li Cheng, Norman J. Dovichi

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Fluorescence detection has produced excellent detection limits in capillary electrophoresis. Laser excitation produces the highest sensitivity detection. lie perceived difficulties associated with the use of the laser has discouraged applications of fluorescence to capillary electrophoresis. In particular, difficulties in wavelength selection limit the choice of chemistry available in capillary clectrophoresis. We report the design of a filter fluorometer based on a compact 75 W xenon arc lamp for capillary electrophoresis; the instrument produces detection limits (3σ) of 20 zeptomol (1 zeptomol = 10-21 mol) for fluorescein and 200 zeptomol for fluorescein labeled amino acids.

Original languageEnglish (US)
Pages (from-to)347-353
Number of pages7
JournalJournal of Chromatography A
Volume652
Issue number2
DOIs
StatePublished - Oct 22 1993

Bibliographical note

Funding Information:
Dionex and the National Sciences and En-gineeringR esearchC ouncil (NSERC) of Canada funded this work. N.J.D. acknowledgesa Steacie fellowship from NSERC.

Fingerprint Dive into the research topics of 'High-efficiency filter fluorometer for capillary electrophoresis and its application to fluorescein thiocarbamyl amino acids'. Together they form a unique fingerprint.

Cite this