Fluorescence detection has produced excellent detection limits in capillary electrophoresis. Laser excitation produces the highest sensitivity detection. lie perceived difficulties associated with the use of the laser has discouraged applications of fluorescence to capillary electrophoresis. In particular, difficulties in wavelength selection limit the choice of chemistry available in capillary clectrophoresis. We report the design of a filter fluorometer based on a compact 75 W xenon arc lamp for capillary electrophoresis; the instrument produces detection limits (3σ) of 20 zeptomol (1 zeptomol = 10-21 mol) for fluorescein and 200 zeptomol for fluorescein labeled amino acids.
Bibliographical noteFunding Information:
Dionex and the National Sciences and En-gineeringR esearchC ouncil (NSERC) of Canada funded this work. N.J.D. acknowledgesa Steacie fellowship from NSERC.