High-Performance Anion-Exchange Chromatography of Oligonucleotides and Oligodeoxynucleotides on Quaternized Polyethylenimine-Coated Zirconia

Clayton McNeff, Peter W Carr

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

The use of porous, quaternized polyethylenimine-coated zirconia particles for the separation of nucleosides, nucleotides, oligonucleotides, and oligodeoxynucleotides is described. Separations of these biomolecules on quatemized PEI-zirconia are presented. Quaternized PEI-zirconia and a quaternary amine functionalized silica are compared chromatographically. Quaternized PEI-zirconia was able to separate oligonucleotides and oligodeoxynucleotides differing in length by a single nucleotide unit It could also separate RNAs of the same length but differing by only one deoxynucleotide. In contrast the silica-based phase was unstable at elevated temperatures (75 °C), and all retention toward anions was lost quite rapidly.

Original languageEnglish (US)
Pages (from-to)2350-2353
Number of pages4
JournalAnalytical chemistry
Volume67
Issue number14
DOIs
StatePublished - Jul 1 1995

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