High-resolution backscatter electron imaging of colloidal gold in LVSEM

Stanley Erlandsen; Y. Chen; C. Frethem; J. Detry; Carol L Wells

doi:10.1046/j.1365-2818.2003.01218.x

High-resolution backscatter electron imaging of colloidal gold in LVSEM

Stanley Erlandsen, Y. Chen, C. Frethem, J. Detry, Carol L Wells

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

High-resolution backscatter electron (BSE) imaging of colloidal gold can be accomplished at low voltage using in-lens or below-the-lens FESEMs equipped with either Autrata-modified yttrium aluminium garnet (YAG) scintillators doped with cerium, or with BSE to secondary electron (SE) conversion plates. The threshold for BSE detection of colloidal gold was 1.8 keV for the YAG detector, and the BSE/SE conversion was sensitive down to 1 keV. Gold particles (6, 12 and 18 nm) have an atomic number of 79 and were clearly distinguished at 500 000x by materials contrast and easily discriminated from cell surfaces coated with platinum with an atomic number of 78. BSE imaging was relatively insensitive to charging, and build up of carbon contamination on the specimen was transparent to the higher energy BSE.

Original language	English (US)
Pages (from-to)	212-218
Number of pages	7
Journal	Journal of Microscopy
Volume	211
Issue number	3
DOIs	https://doi.org/10.1046/j.1365-2818.2003.01218.x
State	Published - Sep 1 2003

Keywords

BSE imaging
Colloidal gold
Contamination
Detector threshold
ExB detector
Low-voltage FESEM
YAG detector

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title = "High-resolution backscatter electron imaging of colloidal gold in LVSEM",

abstract = "High-resolution backscatter electron (BSE) imaging of colloidal gold can be accomplished at low voltage using in-lens or below-the-lens FESEMs equipped with either Autrata-modified yttrium aluminium garnet (YAG) scintillators doped with cerium, or with BSE to secondary electron (SE) conversion plates. The threshold for BSE detection of colloidal gold was 1.8 keV for the YAG detector, and the BSE/SE conversion was sensitive down to 1 keV. Gold particles (6, 12 and 18 nm) have an atomic number of 79 and were clearly distinguished at 500 000x by materials contrast and easily discriminated from cell surfaces coated with platinum with an atomic number of 78. BSE imaging was relatively insensitive to charging, and build up of carbon contamination on the specimen was transparent to the higher energy BSE.",

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AU - Erlandsen, Stanley

AU - Chen, Y.

AU - Frethem, C.

AU - Detry, J.

AU - Wells, Carol L

PY - 2003/9/1

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N2 - High-resolution backscatter electron (BSE) imaging of colloidal gold can be accomplished at low voltage using in-lens or below-the-lens FESEMs equipped with either Autrata-modified yttrium aluminium garnet (YAG) scintillators doped with cerium, or with BSE to secondary electron (SE) conversion plates. The threshold for BSE detection of colloidal gold was 1.8 keV for the YAG detector, and the BSE/SE conversion was sensitive down to 1 keV. Gold particles (6, 12 and 18 nm) have an atomic number of 79 and were clearly distinguished at 500 000x by materials contrast and easily discriminated from cell surfaces coated with platinum with an atomic number of 78. BSE imaging was relatively insensitive to charging, and build up of carbon contamination on the specimen was transparent to the higher energy BSE.

AB - High-resolution backscatter electron (BSE) imaging of colloidal gold can be accomplished at low voltage using in-lens or below-the-lens FESEMs equipped with either Autrata-modified yttrium aluminium garnet (YAG) scintillators doped with cerium, or with BSE to secondary electron (SE) conversion plates. The threshold for BSE detection of colloidal gold was 1.8 keV for the YAG detector, and the BSE/SE conversion was sensitive down to 1 keV. Gold particles (6, 12 and 18 nm) have an atomic number of 79 and were clearly distinguished at 500 000x by materials contrast and easily discriminated from cell surfaces coated with platinum with an atomic number of 78. BSE imaging was relatively insensitive to charging, and build up of carbon contamination on the specimen was transparent to the higher energy BSE.

KW - BSE imaging

KW - Colloidal gold

KW - Contamination

KW - Detector threshold

KW - ExB detector

KW - Low-voltage FESEM

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High-resolution backscatter electron imaging of colloidal gold in LVSEM

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Keywords

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