Abstract
Advances in next-generation sequencing technology have facilitated the discovery of single nucleotide polymorphisms (SNPs). Sequenom-based SNP-typing assays were developed for 1359 maize SNPs identified via comparative next-generation transcriptomic sequencing. Approximately 75% of these SNPs were successfully converted into genetic markers that can be scored reliably and used to generate a SNPbased genetic map by genotyping recombinant inbred lines from the intermated B73 X Mo17 population. The quantitative nature of Sequenom-based SNP assays led to the development of a time- and costefficient strategy to genetically map mutants via quantitative bulked segregant analysis. This strategy was used to rapidly map the loci associated with several dozen recessive mutants. Because a mutant can be mapped using as few as eight multiplexed sets of SNP assays on a bulk of as few as 20 mutant F2 individuals, this strategy is expected to be widely adopted for mapping in many species.
Original language | English (US) |
---|---|
Pages (from-to) | 19-26 |
Number of pages | 8 |
Journal | Genetics |
Volume | 184 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2010 |