Highwire regulates presynaptic BMP signaling essential for synaptic growth

Brian D. McCabe; Sabrina Hom; Hermann Aberle; Richard D. Fetter; Guillermo Marques; Theodore E. Haerry; Hong Wan; Michael B. O'Connor; Corey S. Goodman; A. Pejmun Haghighi

doi:10.1016/S0896-6273(04)00073-X

Highwire regulates presynaptic BMP signaling essential for synaptic growth

Brian D. McCabe, Sabrina Hom, Hermann Aberle, Richard D. Fetter, Guillermo Marques, Theodore E. Haerry, Hong Wan, Michael B. O'Connor, Corey S. Goodman, A. Pejmun Haghighi

Research output: Contribution to journal › Article › peer-review

179 Scopus citations

Abstract

Highwire (Hiw), a putative RING finger E3 ubiquitin ligase, negatively regulates synaptic growth at the neuromuscular junction (NMJ) in Drosophila. hiw mutants have dramatically larger synaptic size and increased numbers of synaptic boutons. Here we show that Hiw binds to the Smad protein Medea (Med). Med is part of a presynaptic bone morphogenetic protein (BMP) signaling cascade consisting of three receptor subunits, Wit, Tkv, and Sax, in addition to the Smad transcription factor Mad. When compared to wild-type, mutants of BMP signaling components have smaller NMJ size, reduced neurotransmitter release, and aberrant synaptic ultrastructure. BMP signaling mutants suppress the excessive synaptic growth in hiw mutants. Activation of BMP signaling, which in wild-type does not cause additional growth, in hiw mutants does lead to further synaptic expansion. These results reveal a balance between positive BMP signaling and negative regulation by Highwire, governing the growth of neuromuscular synapses.

Original language	English (US)
Pages (from-to)	891-905
Number of pages	15
Journal	Neuron
Volume	41
Issue number	6
DOIs	https://doi.org/10.1016/S0896-6273(04)00073-X
State	Published - Mar 25 2004

Bibliographical note

Funding Information:
We are grateful to the Bloomington stock center, Aaron DiAntonio, and Laurel Raftery for supplying stocks. We thank S. Elledge for reagents. We thank Dorit Parnas, Cynthia Hsu, and Ben Sowers for their contributions. We are grateful to Clara Henriquez and Beth Blankemier for technical assistance. B.D.M was supported by a Wellcome Trust Prize Traveling fellowship. A.P.H. was supported by the Canadian Natural Sciences and Engineering Research Council. R.D.F. is a research specialist, and M.B.O. is an investigator with the Howard Hughes Medical Institute.

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title = "Highwire regulates presynaptic BMP signaling essential for synaptic growth",

abstract = "Highwire (Hiw), a putative RING finger E3 ubiquitin ligase, negatively regulates synaptic growth at the neuromuscular junction (NMJ) in Drosophila. hiw mutants have dramatically larger synaptic size and increased numbers of synaptic boutons. Here we show that Hiw binds to the Smad protein Medea (Med). Med is part of a presynaptic bone morphogenetic protein (BMP) signaling cascade consisting of three receptor subunits, Wit, Tkv, and Sax, in addition to the Smad transcription factor Mad. When compared to wild-type, mutants of BMP signaling components have smaller NMJ size, reduced neurotransmitter release, and aberrant synaptic ultrastructure. BMP signaling mutants suppress the excessive synaptic growth in hiw mutants. Activation of BMP signaling, which in wild-type does not cause additional growth, in hiw mutants does lead to further synaptic expansion. These results reveal a balance between positive BMP signaling and negative regulation by Highwire, governing the growth of neuromuscular synapses.",

author = "McCabe, {Brian D.} and Sabrina Hom and Hermann Aberle and Fetter, {Richard D.} and Guillermo Marques and Haerry, {Theodore E.} and Hong Wan and O'Connor, {Michael B.} and Goodman, {Corey S.} and Haghighi, {A. Pejmun}",

note = "Funding Information: We are grateful to the Bloomington stock center, Aaron DiAntonio, and Laurel Raftery for supplying stocks. We thank S. Elledge for reagents. We thank Dorit Parnas, Cynthia Hsu, and Ben Sowers for their contributions. We are grateful to Clara Henriquez and Beth Blankemier for technical assistance. B.D.M was supported by a Wellcome Trust Prize Traveling fellowship. A.P.H. was supported by the Canadian Natural Sciences and Engineering Research Council. R.D.F. is a research specialist, and M.B.O. is an investigator with the Howard Hughes Medical Institute. ",

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AU - Haerry, Theodore E.

AU - Wan, Hong

AU - O'Connor, Michael B.

AU - Goodman, Corey S.

AU - Haghighi, A. Pejmun

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PY - 2004/3/25

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N2 - Highwire (Hiw), a putative RING finger E3 ubiquitin ligase, negatively regulates synaptic growth at the neuromuscular junction (NMJ) in Drosophila. hiw mutants have dramatically larger synaptic size and increased numbers of synaptic boutons. Here we show that Hiw binds to the Smad protein Medea (Med). Med is part of a presynaptic bone morphogenetic protein (BMP) signaling cascade consisting of three receptor subunits, Wit, Tkv, and Sax, in addition to the Smad transcription factor Mad. When compared to wild-type, mutants of BMP signaling components have smaller NMJ size, reduced neurotransmitter release, and aberrant synaptic ultrastructure. BMP signaling mutants suppress the excessive synaptic growth in hiw mutants. Activation of BMP signaling, which in wild-type does not cause additional growth, in hiw mutants does lead to further synaptic expansion. These results reveal a balance between positive BMP signaling and negative regulation by Highwire, governing the growth of neuromuscular synapses.

AB - Highwire (Hiw), a putative RING finger E3 ubiquitin ligase, negatively regulates synaptic growth at the neuromuscular junction (NMJ) in Drosophila. hiw mutants have dramatically larger synaptic size and increased numbers of synaptic boutons. Here we show that Hiw binds to the Smad protein Medea (Med). Med is part of a presynaptic bone morphogenetic protein (BMP) signaling cascade consisting of three receptor subunits, Wit, Tkv, and Sax, in addition to the Smad transcription factor Mad. When compared to wild-type, mutants of BMP signaling components have smaller NMJ size, reduced neurotransmitter release, and aberrant synaptic ultrastructure. BMP signaling mutants suppress the excessive synaptic growth in hiw mutants. Activation of BMP signaling, which in wild-type does not cause additional growth, in hiw mutants does lead to further synaptic expansion. These results reveal a balance between positive BMP signaling and negative regulation by Highwire, governing the growth of neuromuscular synapses.

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Highwire regulates presynaptic BMP signaling essential for synaptic growth

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Highwire regulates presynaptic BMP signaling essential for synaptic growth

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