TY - JOUR
T1 - Histochemical and immunohistochemical characterization of cells constituting the giant cell tumor of bone
AU - Clohisy, D. R.
AU - Vorlicky, L.
AU - Oegema, T. R.
AU - Snover, D.
AU - Thompson, Roby C
PY - 1993
Y1 - 1993
N2 - Enzymatic activity and cell membrane proteins were characterized in cells from five giant cell tumors of bone (GCTs). Napthyl alpha esterase (NAE) and acid phosphatase (AP) activity was noted within both the mononuclear and multinucleated cells of each tumor. In each tumor, all mononucleated cell populations displayed tartrate-sensitive AP activity, whereas the multinucleated cell populations demonstrated variable expression of tartrate- sensitive and tartrate-resistant AP activity. Analysis of cell membrane proteins included attempts at immunodetection of mannose receptor, OKM-1 antigen (OKM-1a), colony-stimulating factor-1 receptor (CSF-1r), and platelet-derived growth factor receptor (PDGFr). None of these membrane antigens were elicited on multinucleated cells. In contrast, the mannose receptor, OKM-1a, and PDGFr all were detected on the mononucleated cells within each tumor. These data demonstrate that a population of mononucleated, not multinucleated cells, expresses features unique to mature mononuclear phagocytes and establishes the presence of a membrane receptor, PDGFr, associated with mitogenesis of mesenchymal cells.
AB - Enzymatic activity and cell membrane proteins were characterized in cells from five giant cell tumors of bone (GCTs). Napthyl alpha esterase (NAE) and acid phosphatase (AP) activity was noted within both the mononuclear and multinucleated cells of each tumor. In each tumor, all mononucleated cell populations displayed tartrate-sensitive AP activity, whereas the multinucleated cell populations demonstrated variable expression of tartrate- sensitive and tartrate-resistant AP activity. Analysis of cell membrane proteins included attempts at immunodetection of mannose receptor, OKM-1 antigen (OKM-1a), colony-stimulating factor-1 receptor (CSF-1r), and platelet-derived growth factor receptor (PDGFr). None of these membrane antigens were elicited on multinucleated cells. In contrast, the mannose receptor, OKM-1a, and PDGFr all were detected on the mononucleated cells within each tumor. These data demonstrate that a population of mononucleated, not multinucleated cells, expresses features unique to mature mononuclear phagocytes and establishes the presence of a membrane receptor, PDGFr, associated with mitogenesis of mesenchymal cells.
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U2 - 10.1097/00003086-199302000-00041
DO - 10.1097/00003086-199302000-00041
M3 - Article
C2 - 8448954
AN - SCOPUS:0027522577
SN - 0009-921X
VL - 287
SP - 259
EP - 265
JO - Clinical orthopaedics and related research
JF - Clinical orthopaedics and related research
ER -