To the Editor: Pomerantz et al. (Dec. 24 issue)1 described the isolation of human immunodeficiency virus (HIV) from the retina.1 We have also detected HIV in the retina and other ocular tissues from three patients with the acquired immunodeficiency syndrome (AIDS).2 All three had cytomegalovirus retinitis and had received intravitreal injections of ganciclovir.3 Two of the three had received systemic zidovudine. Homogenized retinal tissue was cultured with phytohemagglutinin-stimulated peripheral-blood leukocytes obtained from healthy HIV-seronegative donors. The culture supernatants were sampled every seven days for HIV-1 p24 antigen by enzyme immunoassay. All three retinas examined were positive for p24 antigen within.