Hormonal regulation of serum alpha1-antitrypsin and hepatic alpha1-antitrypsin mRNA in rats

Sarah J Schwarzenberg, Harvey L. Sharp, Susan A Berry, Robin D. Manthei, Steven Seelig

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

We evaluated the effects of pituitary dependent hormones on alpha1-antitrypsin in male rats. Hepatic alpha1-antitrypsin mRNA was measured by in vitro translation and by specific hybridization with a mouse cDNA alpha1-antitrypsin probe. Hypophysectomy caused a 50-75% decrease in serum elastase inhibitory capacity (measuring functional alpha1-antitrypsin) and hepatic alpha1-antitrypsin mRNA content. In hypophysectomized animals, no increase in elastase inhibitory capacity or alpha1-antitrypsin mRNA levels by translation was found when met-human growth hormone alone or corticosterone, dihydrotestosterone and thyroxine were given together. Growth hormone increased alpha1-antitrypsin mRNA by hybridization to a small extent. Addition of growth hormone to the combination of corticosterone, dihydrotestosterone, and thyroxine increased serum elastase inhibitory capacity and alpha1-antitrypsin mRNA. We conclude that growth hormone acts synergistically with the other pituitary dependent hormones to regulate serum and hepatic mRNA levels of alpha1-antitrypsin.

Original languageEnglish (US)
Pages (from-to)936-941
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume147
Issue number3
DOIs
StatePublished - Sep 30 1987

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