We evaluated the effects of pituitary dependent hormones on alpha1-antitrypsin in male rats. Hepatic alpha1-antitrypsin mRNA was measured by in vitro translation and by specific hybridization with a mouse cDNA alpha1-antitrypsin probe. Hypophysectomy caused a 50-75% decrease in serum elastase inhibitory capacity (measuring functional alpha1-antitrypsin) and hepatic alpha1-antitrypsin mRNA content. In hypophysectomized animals, no increase in elastase inhibitory capacity or alpha1-antitrypsin mRNA levels by translation was found when met-human growth hormone alone or corticosterone, dihydrotestosterone and thyroxine were given together. Growth hormone increased alpha1-antitrypsin mRNA by hybridization to a small extent. Addition of growth hormone to the combination of corticosterone, dihydrotestosterone, and thyroxine increased serum elastase inhibitory capacity and alpha1-antitrypsin mRNA. We conclude that growth hormone acts synergistically with the other pituitary dependent hormones to regulate serum and hepatic mRNA levels of alpha1-antitrypsin.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Sep 30 1987|