Hormonal regulation of serum alpha1-antitrypsin and hepatic alpha1-antitrypsin mRNA in rats

Sarah J Schwarzenberg; Harvey L. Sharp; Susan A Berry; Robin D. Manthei; Steven Seelig

doi:10.1016/S0006-291X(87)80160-2

Hormonal regulation of serum alpha₁-antitrypsin and hepatic alpha₁-antitrypsin mRNA in rats

Sarah J Schwarzenberg, Harvey L. Sharp, Susan A Berry, Robin D. Manthei, Steven Seelig

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

We evaluated the effects of pituitary dependent hormones on alpha₁-antitrypsin in male rats. Hepatic alpha₁-antitrypsin mRNA was measured by in vitro translation and by specific hybridization with a mouse cDNA alpha₁-antitrypsin probe. Hypophysectomy caused a 50-75% decrease in serum elastase inhibitory capacity (measuring functional alpha₁-antitrypsin) and hepatic alpha₁-antitrypsin mRNA content. In hypophysectomized animals, no increase in elastase inhibitory capacity or alpha₁-antitrypsin mRNA levels by translation was found when met-human growth hormone alone or corticosterone, dihydrotestosterone and thyroxine were given together. Growth hormone increased alpha₁-antitrypsin mRNA by hybridization to a small extent. Addition of growth hormone to the combination of corticosterone, dihydrotestosterone, and thyroxine increased serum elastase inhibitory capacity and alpha₁-antitrypsin mRNA. We conclude that growth hormone acts synergistically with the other pituitary dependent hormones to regulate serum and hepatic mRNA levels of alpha₁-antitrypsin.

Original language	English (US)
Pages (from-to)	936-941
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	147
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(87)80160-2
State	Published - Sep 30 1987

Bibliographical note

Funding Information:
Acknowledgements: This work was supported by an American Gastroenterology Association Interdisciplinary Training Supplement Award, a Cystic Fibrosis Training Fellowship, an American Liver Foundation Postdoctoral Fellowship, and National Research Service Award AM07420 (SJS),

Funding Information:
National Institute of Health AM34931 (ILLS), National Research Service Award AM07322 (SAB), and National Institute of Health Grant AM32817 and Basil O'Connor Starter Research Grant 5-434

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title = "Hormonal regulation of serum alpha1-antitrypsin and hepatic alpha1-antitrypsin mRNA in rats",

abstract = "We evaluated the effects of pituitary dependent hormones on alpha1-antitrypsin in male rats. Hepatic alpha1-antitrypsin mRNA was measured by in vitro translation and by specific hybridization with a mouse cDNA alpha1-antitrypsin probe. Hypophysectomy caused a 50-75% decrease in serum elastase inhibitory capacity (measuring functional alpha1-antitrypsin) and hepatic alpha1-antitrypsin mRNA content. In hypophysectomized animals, no increase in elastase inhibitory capacity or alpha1-antitrypsin mRNA levels by translation was found when met-human growth hormone alone or corticosterone, dihydrotestosterone and thyroxine were given together. Growth hormone increased alpha1-antitrypsin mRNA by hybridization to a small extent. Addition of growth hormone to the combination of corticosterone, dihydrotestosterone, and thyroxine increased serum elastase inhibitory capacity and alpha1-antitrypsin mRNA. We conclude that growth hormone acts synergistically with the other pituitary dependent hormones to regulate serum and hepatic mRNA levels of alpha1-antitrypsin.",

author = "Schwarzenberg, {Sarah J} and Sharp, {Harvey L.} and Berry, {Susan A} and Manthei, {Robin D.} and Steven Seelig",

note = "Funding Information: Acknowledgements: This work was supported by an American Gastroenterology Association Interdisciplinary Training Supplement Award, a Cystic Fibrosis Training Fellowship, an American Liver Foundation Postdoctoral Fellowship, and National Research Service Award AM07420 (SJS), Funding Information: National Institute of Health AM34931 (ILLS), National Research Service Award AM07322 (SAB), and National Institute of Health Grant AM32817 and Basil O'Connor Starter Research Grant 5-434",

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AU - Manthei, Robin D.

AU - Seelig, Steven

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N2 - We evaluated the effects of pituitary dependent hormones on alpha1-antitrypsin in male rats. Hepatic alpha1-antitrypsin mRNA was measured by in vitro translation and by specific hybridization with a mouse cDNA alpha1-antitrypsin probe. Hypophysectomy caused a 50-75% decrease in serum elastase inhibitory capacity (measuring functional alpha1-antitrypsin) and hepatic alpha1-antitrypsin mRNA content. In hypophysectomized animals, no increase in elastase inhibitory capacity or alpha1-antitrypsin mRNA levels by translation was found when met-human growth hormone alone or corticosterone, dihydrotestosterone and thyroxine were given together. Growth hormone increased alpha1-antitrypsin mRNA by hybridization to a small extent. Addition of growth hormone to the combination of corticosterone, dihydrotestosterone, and thyroxine increased serum elastase inhibitory capacity and alpha1-antitrypsin mRNA. We conclude that growth hormone acts synergistically with the other pituitary dependent hormones to regulate serum and hepatic mRNA levels of alpha1-antitrypsin.

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