Host cell-specific expression of a p44 epitope by the human granulocytic ehrlichiosis agent

Steven D. Jauron; Curtis M. Nelson; Volker Fingerle; M. Dana Ravyn; Jesse L. Goodman; Russell C. Johnson; Ruth Lobentanzer; Bettina Wilske; Ulrike G Munderloh

doi:10.1086/324428

Host cell-specific expression of a p44 epitope by the human granulocytic ehrlichiosis agent

Steven D. Jauron, Curtis M. Nelson, Volker Fingerle, M. Dana Ravyn, Jesse L. Goodman, Russell C. Johnson, Ruth Lobentanzer, Bettina Wilske, Ulrike G Munderloh

Entomology

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Abstract

The human granulocytic ehrlichiosis agent (HGEa) survives extreme differences between ticks and humans possibly by use of differential expression of specific antigens for survival in different hosts. The role of the immunodominant p44 antigens is unknown. In this study HGEa cultured in human or tick cells was probed with human mouse and hamster serum and with monoclonal antibodies (MAbs). p44 antigens were strongly expressed in human HL-60 cells but were strikingly reduced in tick cells. In HGEa alternately grown in HL-60 or tick cells a p44 epitope recognized by MAb R5E4 was expressed in human but not tick cells. This was not a temperature effect because incubation of infected tick cells at 37°C did not induce expression of the p44 epitope. The p44 antigen predominates in human but not tick cells and may be involved in regulatory changes that mediate survival of the HGEa by immune modulation after tick transmission.

Original language	English (US)
Pages (from-to)	1445-1450
Number of pages	6
Journal	Journal of Infectious Diseases
Volume	184
Issue number	11
DOIs	https://doi.org/10.1086/324428
State	Published - Dec 2001

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title = "Host cell-specific expression of a p44 epitope by the human granulocytic ehrlichiosis agent",

abstract = "The human granulocytic ehrlichiosis agent (HGEa) survives extreme differences between ticks and humans possibly by use of differential expression of specific antigens for survival in different hosts. The role of the immunodominant p44 antigens is unknown. In this study HGEa cultured in human or tick cells was probed with human mouse and hamster serum and with monoclonal antibodies (MAbs). p44 antigens were strongly expressed in human HL-60 cells but were strikingly reduced in tick cells. In HGEa alternately grown in HL-60 or tick cells a p44 epitope recognized by MAb R5E4 was expressed in human but not tick cells. This was not a temperature effect because incubation of infected tick cells at 37°C did not induce expression of the p44 epitope. The p44 antigen predominates in human but not tick cells and may be involved in regulatory changes that mediate survival of the HGEa by immune modulation after tick transmission.",

author = "Jauron, {Steven D.} and Nelson, {Curtis M.} and Volker Fingerle and Ravyn, {M. Dana} and Goodman, {Jesse L.} and Johnson, {Russell C.} and Ruth Lobentanzer and Bettina Wilske and Munderloh, {Ulrike G}",

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AU - Jauron, Steven D.

AU - Nelson, Curtis M.

AU - Fingerle, Volker

AU - Ravyn, M. Dana

AU - Goodman, Jesse L.

AU - Johnson, Russell C.

AU - Lobentanzer, Ruth

AU - Wilske, Bettina

AU - Munderloh, Ulrike G

PY - 2001/12

Y1 - 2001/12

N2 - The human granulocytic ehrlichiosis agent (HGEa) survives extreme differences between ticks and humans possibly by use of differential expression of specific antigens for survival in different hosts. The role of the immunodominant p44 antigens is unknown. In this study HGEa cultured in human or tick cells was probed with human mouse and hamster serum and with monoclonal antibodies (MAbs). p44 antigens were strongly expressed in human HL-60 cells but were strikingly reduced in tick cells. In HGEa alternately grown in HL-60 or tick cells a p44 epitope recognized by MAb R5E4 was expressed in human but not tick cells. This was not a temperature effect because incubation of infected tick cells at 37°C did not induce expression of the p44 epitope. The p44 antigen predominates in human but not tick cells and may be involved in regulatory changes that mediate survival of the HGEa by immune modulation after tick transmission.

AB - The human granulocytic ehrlichiosis agent (HGEa) survives extreme differences between ticks and humans possibly by use of differential expression of specific antigens for survival in different hosts. The role of the immunodominant p44 antigens is unknown. In this study HGEa cultured in human or tick cells was probed with human mouse and hamster serum and with monoclonal antibodies (MAbs). p44 antigens were strongly expressed in human HL-60 cells but were strikingly reduced in tick cells. In HGEa alternately grown in HL-60 or tick cells a p44 epitope recognized by MAb R5E4 was expressed in human but not tick cells. This was not a temperature effect because incubation of infected tick cells at 37°C did not induce expression of the p44 epitope. The p44 antigen predominates in human but not tick cells and may be involved in regulatory changes that mediate survival of the HGEa by immune modulation after tick transmission.

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Host cell-specific expression of a p44 epitope by the human granulocytic ehrlichiosis agent

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