HPLC Analysis of the Phenylisothiocyanate (PITC) Derivatives of Taurine from Physiologic Samples

Shirley E. Lippincott; Aaron L. Friedman; Frank L. Siegel; Rita M. Pityer; Russell W. Chesney

doi:10.1080/07315724.1988.10720265

HPLC Analysis of the Phenylisothiocyanate (PITC) Derivatives of Taurine from Physiologic Samples

Shirley E. Lippincott, Aaron L. Friedman, Frank L. Siegel, Rita M. Pityer, Russell W. Chesney

Pediatrics - Administration

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Taurine is the major free intracellular amino acid. It has become the focus of study by many as a conjugator of bile and as a neurotransmitter and intracellular messenger. In this report we document a technique for measuring taurine in physiologic samples which is rapid, reproducible, and accurate. Any physiologic sample is first derivatized with phenylisothiocyanate (PITC) and separated by reverse phase HPLC, and then taurine is detected by UV at 254 nm. The advantages of this technique for the measurement of taurine are accuracy, small sample size, and reproducibility, and with an automated system many samples can be analyzed.

Original language	English (US)
Pages (from-to)	491-497
Number of pages	7
Journal	Journal of the American College of Nutrition
Volume	7
Issue number	6
DOIs	https://doi.org/10.1080/07315724.1988.10720265
State	Published - Dec 1 1988

Bibliographical note

Funding Information:
We acknowledge the financial support of the National Institute of Health ROI AM31682, The Graduate School of the University of Wisconsin, and Baxter-Travenol, Inc.

Keywords

HPLC
PITC derivatives
Physiologic samples
Taurine

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10.1080/07315724.1988.10720265

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title = "HPLC Analysis of the Phenylisothiocyanate (PITC) Derivatives of Taurine from Physiologic Samples",

abstract = "Taurine is the major free intracellular amino acid. It has become the focus of study by many as a conjugator of bile and as a neurotransmitter and intracellular messenger. In this report we document a technique for measuring taurine in physiologic samples which is rapid, reproducible, and accurate. Any physiologic sample is first derivatized with phenylisothiocyanate (PITC) and separated by reverse phase HPLC, and then taurine is detected by UV at 254 nm. The advantages of this technique for the measurement of taurine are accuracy, small sample size, and reproducibility, and with an automated system many samples can be analyzed.",

keywords = "HPLC, PITC derivatives, Physiologic samples, Taurine",

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note = "Funding Information: We acknowledge the financial support of the National Institute of Health ROI AM31682, The Graduate School of the University of Wisconsin, and Baxter-Travenol, Inc.",

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AU - Lippincott, Shirley E.

AU - Friedman, Aaron L.

AU - Siegel, Frank L.

AU - Pityer, Rita M.

AU - Chesney, Russell W.

N1 - Funding Information: We acknowledge the financial support of the National Institute of Health ROI AM31682, The Graduate School of the University of Wisconsin, and Baxter-Travenol, Inc.

PY - 1988/12/1

Y1 - 1988/12/1

N2 - Taurine is the major free intracellular amino acid. It has become the focus of study by many as a conjugator of bile and as a neurotransmitter and intracellular messenger. In this report we document a technique for measuring taurine in physiologic samples which is rapid, reproducible, and accurate. Any physiologic sample is first derivatized with phenylisothiocyanate (PITC) and separated by reverse phase HPLC, and then taurine is detected by UV at 254 nm. The advantages of this technique for the measurement of taurine are accuracy, small sample size, and reproducibility, and with an automated system many samples can be analyzed.

AB - Taurine is the major free intracellular amino acid. It has become the focus of study by many as a conjugator of bile and as a neurotransmitter and intracellular messenger. In this report we document a technique for measuring taurine in physiologic samples which is rapid, reproducible, and accurate. Any physiologic sample is first derivatized with phenylisothiocyanate (PITC) and separated by reverse phase HPLC, and then taurine is detected by UV at 254 nm. The advantages of this technique for the measurement of taurine are accuracy, small sample size, and reproducibility, and with an automated system many samples can be analyzed.

KW - HPLC

KW - PITC derivatives

KW - Physiologic samples

KW - Taurine

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JF - Journal of the American College of Nutrition

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ER -

HPLC Analysis of the Phenylisothiocyanate (PITC) Derivatives of Taurine from Physiologic Samples

Abstract

Bibliographical note

Keywords

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