HPLC Analysis of the Phenylisothiocyanate (PITC) Derivatives of Taurine from Physiologic Samples

Shirley E. Lippincott, Aaron L. Friedman, Frank L. Siegel, Rita M. Pityer, Russell W. Chesney

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Taurine is the major free intracellular amino acid. It has become the focus of study by many as a conjugator of bile and as a neurotransmitter and intracellular messenger. In this report we document a technique for measuring taurine in physiologic samples which is rapid, reproducible, and accurate. Any physiologic sample is first derivatized with phenylisothiocyanate (PITC) and separated by reverse phase HPLC, and then taurine is detected by UV at 254 nm. The advantages of this technique for the measurement of taurine are accuracy, small sample size, and reproducibility, and with an automated system many samples can be analyzed.

Original languageEnglish (US)
Pages (from-to)491-497
Number of pages7
JournalJournal of the American College of Nutrition
Volume7
Issue number6
DOIs
StatePublished - Dec 1 1988

Bibliographical note

Funding Information:
We acknowledge the financial support of the National Institute of Health ROI AM31682, The Graduate School of the University of Wisconsin, and Baxter-Travenol, Inc.

Keywords

  • HPLC
  • PITC derivatives
  • Physiologic samples
  • Taurine

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