TY - JOUR
T1 - Human hepatic stellate cell line (LX-2) exhibits characteristics of bone marrow-derived mesenchymal stem cells
AU - Castilho-Fernandes, Andrielle
AU - de Almeida, Danilo Candido
AU - Fontes, Aparecida Maria
AU - Melo, Fernanda Ursoli Ferreira
AU - Picanço-Castro, Virgínia
AU - Freitas, Marcela Cristina
AU - Orellana, Maristela D.
AU - Palma, Patricia V.B.
AU - Hackett, Perry B.
AU - Friedman, Scott L.
AU - Covas, Dimas Tadeu
N1 - Funding Information:
We would like to thank, Dr. Dario Campana (Department of Pediatrics, University of Tennessee College of Medicine, Memphis, TN) for kindly providing HBMS cell line. Also we thank Lucas E. B. Souza for providing technical support on Xenogen In Vivo Imaging System (IVIS). This work was supported by FAPESP , FINEP and INCTC .
PY - 2011/12
Y1 - 2011/12
N2 - The LX-2 cell line has characteristics of hepatic stellate cells (HSCs), which are considered pericytes of the hepatic microcirculatory system. Recent studies have suggested that HSCs might have mesenchymal origin. We have performed an extensive characterization of the LX-2 cells and have compared their features with those of mesenchymal cells. Our data show that LX-2 cells have a phenotype resembling activated HSCs as well as bone marrow-derived mesenchymal stem cells (BM-MSCs). Our immunophenotypic analysis showed that LX-2 cells are positive for activated HSC markers (αSMA, GFAP, nestin and CD271) and classical mesenchymal makers (CD105, CD44, CD29, CD13, CD90, HLA class-I, CD73, CD49e, CD166 and CD146) but negative for the endothelial marker CD31 and endothelial progenitor cell marker CD133 as well as hematopoietic markers (CD45 and CD34). LX-2 cells also express the same transcripts found in immortalized and primary BM-MSCs (vimentin, annexin 5, collagen 1A, NG2 and CD140b), although at different levels. We show that LX-2 cells are capable to differentiate into multilineage mesenchymal cells in vitro and can stimulate new blood vessel formation in vivo. LX-2 cells appear not to possess tumorigenic potential. Thus, the LX-2 cell line behaves as a multipotent cell line with similarity to BM-MSCs. This line should be useful for further studies to elucidate liver regeneration mechanisms and be the foundation for development of hepatic cell-based therapies.
AB - The LX-2 cell line has characteristics of hepatic stellate cells (HSCs), which are considered pericytes of the hepatic microcirculatory system. Recent studies have suggested that HSCs might have mesenchymal origin. We have performed an extensive characterization of the LX-2 cells and have compared their features with those of mesenchymal cells. Our data show that LX-2 cells have a phenotype resembling activated HSCs as well as bone marrow-derived mesenchymal stem cells (BM-MSCs). Our immunophenotypic analysis showed that LX-2 cells are positive for activated HSC markers (αSMA, GFAP, nestin and CD271) and classical mesenchymal makers (CD105, CD44, CD29, CD13, CD90, HLA class-I, CD73, CD49e, CD166 and CD146) but negative for the endothelial marker CD31 and endothelial progenitor cell marker CD133 as well as hematopoietic markers (CD45 and CD34). LX-2 cells also express the same transcripts found in immortalized and primary BM-MSCs (vimentin, annexin 5, collagen 1A, NG2 and CD140b), although at different levels. We show that LX-2 cells are capable to differentiate into multilineage mesenchymal cells in vitro and can stimulate new blood vessel formation in vivo. LX-2 cells appear not to possess tumorigenic potential. Thus, the LX-2 cell line behaves as a multipotent cell line with similarity to BM-MSCs. This line should be useful for further studies to elucidate liver regeneration mechanisms and be the foundation for development of hepatic cell-based therapies.
KW - Hepatic stellate cell line
KW - LX-2
KW - Mesenchymal stem cells
KW - Pericytes
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U2 - 10.1016/j.yexmp.2011.09.002
DO - 10.1016/j.yexmp.2011.09.002
M3 - Article
C2 - 21930125
AN - SCOPUS:80053505418
SN - 0014-4800
VL - 91
SP - 664
EP - 672
JO - Experimental and Molecular Pathology
JF - Experimental and Molecular Pathology
IS - 3
ER -
