The quantitative relationship between sulfate reducing bacteria (SRB) and hydrogen (H2) production from sulfate (SO42-) and ferrous [Fe(II)] enriched wastewater was investigated. Both Fe(II) (0-11,600 mg/L) and SO42- (0-20,000 mg/L) improved the H 2 production efficiency from wastewater. The H2 yields were increased up to 1.9 mol H2/mol glucose in 580-1750 mg Fe(II)/L and 1000-3000 mg SO42-/L enriched wastewater at pH 5.8-6.2. Quantitative Fluorescence In Situ Hybridization (FISH) analyses revealed that the specific sulfate reducing activities (SSRA) were increased from 0.08 and 0.06 to 0.16 and 0.21 g TS/g SRB h in response to variations in sulfate concentration from 300-20,000 mg/L at pH 5.8 and 6.2, respectively. H2 production was not influenced by low SSRA (≤0.1 g TS/g SRB h), which was independent of pH variation. The results demonstrated that the SSRA and Fe(II) concentration can significantly influence on the biological H 2 production from SO42- and Fe(II) containing wastewater.
Bibliographical noteFunding Information:
This work was supported by the Senior Researchers National Research Foundation of Korea , 2010-0026904 ), the Brain Korea-21 (BK-21) program of the Ministry of Education, the Global Research Laboratory(GRL) Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (MEST) of Korea (Grant number: 2010-00248 ), the Korea Institute of Energy Research, and the Korea Ministry of Environment (MOE) as Human Resource Development Project for Energy from Waste & Recycling.
- Quantitative SRB