TY - JOUR
T1 - Hypotonicity activates a native chloride current in Xenopus oocytes
AU - Ackerman, Michael J.
AU - Wickman, Kevin D.
AU - Clapham, David E.
PY - 1994/2
Y1 - 1994/2
N2 - Xenopus oocytes are frequently utilized for in vivo expression of cellular proteins, especially ion channel proteins. A thorough understanding of the endogenous conductances and their regulation is paramount for proper characterization of expressed channel proteins. Here we detail a novel chloride current (I(Cl.swell)) responsive to hypotonicity in Xenopus oocytes using the two-electrode voltage clamp technique. Reducing the extracellular osmolarity by 50% elicited a calcium-independent chloride current having an anion conductivity sequence identical with swelling-induced chloride currents observed in epithelial cells. The hypotonicity-activated current was blocked by chloride channel blockers, trivalent lanthanides, and nucleotides. G- protein, cAMP-PKA, and arachidonic acid signaling cascades were not involved in I(Cl.swell) activation. I(Cl.swell) is distinct from both stretch- activated nonselective cation channels and the calcium-activated chloride current in oocytes and may play a critical role in volume regulation in Xenopus oocytes.
AB - Xenopus oocytes are frequently utilized for in vivo expression of cellular proteins, especially ion channel proteins. A thorough understanding of the endogenous conductances and their regulation is paramount for proper characterization of expressed channel proteins. Here we detail a novel chloride current (I(Cl.swell)) responsive to hypotonicity in Xenopus oocytes using the two-electrode voltage clamp technique. Reducing the extracellular osmolarity by 50% elicited a calcium-independent chloride current having an anion conductivity sequence identical with swelling-induced chloride currents observed in epithelial cells. The hypotonicity-activated current was blocked by chloride channel blockers, trivalent lanthanides, and nucleotides. G- protein, cAMP-PKA, and arachidonic acid signaling cascades were not involved in I(Cl.swell) activation. I(Cl.swell) is distinct from both stretch- activated nonselective cation channels and the calcium-activated chloride current in oocytes and may play a critical role in volume regulation in Xenopus oocytes.
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M3 - Article
C2 - 8189203
AN - SCOPUS:0027955784
SN - 0022-1295
VL - 103
SP - 153
EP - 179
JO - Journal of General Physiology
JF - Journal of General Physiology
IS - 2
ER -