Identification and characterization of a fast-neutron-induced mutant with elevated seed protein content in soybean

Elizabeth M. Prenger, Alexandra Ostezan, M. A.Rouf Mian, Robert M. Stupar, Travis Glenn, Zenglu Li

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Key message: Protein content of soybean is critical for utility of soybean meal. A fast-neutron-induced deletion on chromosome 12 was found to be associated with increased protein content. Abstract: Soybean seed composition affects the utility of soybean, and improving seed composition is an essential breeding goal. Fast neutron radiation introduces genomic mutations resulting in novel variation for traits of interest. Two elite soybean lines were irradiated with fast neutrons and screened for altered seed composition. Twenty-three lines with altered protein, oil, or sucrose content were selected based on near-infrared spectroscopy data from five environments and yield tested at five locations. Mutants with significantly increased protein averaged 19.1–36.8 g kg−1 more protein than the parents across 10 environments. Comparative genomic hybridization (CGH) identified putative mutations in a mutant, G15FN-12, that has 36.8 g kg−1 higher protein than the parent genotype, and whole genome sequencing (WGS) of the mutant has confirmed these mutations. An F2:3 population was developed from G15FN-12 to determine association between genomic changes and increased protein content. Bulked segregant analysis of the population using the SoySNP50K BeadChip identified a CGH- and WGS-confirmed deletion on chromosome 12 to be responsible for elevated protein content. The population was genotyped using a KASP marker designed at the mutation region, and significant association (P < 0.0001) between the deletion on chromosome 12 and elevated protein content was observed and confirmed in the F3:4 generation. The F2 segregants homozygous for the deletion averaged 27 g kg−1 higher seed protein and 8 g kg−1 lower oil than homozygous wild-type segregants. Mutants with altered seed composition are a new resource for gene function studies and provide elite materials for genetic improvement of seed composition.

Original languageEnglish (US)
Pages (from-to)2965-2983
Number of pages19
JournalTheoretical and Applied Genetics
Issue number11
StatePublished - Nov 1 2019

Bibliographical note

Funding Information:
We thank the United Soybean Board and the John Ingle Innovation in Plant Breeding Award for funding this research. Thanks to Dale Wood, Earl Baxter, Brice Wilson, Jeremy Nation, Greg Gokalp, Ricky Zoller, Tatyana Nienow, Troy Kieran, and Swarnali Louha from University of Georgia, and Adrian Stec and Jean-Michel Michno from University of Minnesota for their technical support. Thanks to Blair Buckley for growing the yield trial at Bossier, LA. Thanks to Drs. Henry Nguyen and Tri Vuong from University of Missouri for analyzing the sugar content.

Publisher Copyright:
© 2019, Springer-Verlag GmbH Germany, part of Springer Nature.

Copyright 2019 Elsevier B.V., All rights reserved.


  • Chromosome 12
  • Deletion
  • Fast neutrons
  • Mutant
  • Protein content
  • Soybeans

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