Identification and isolation of somatic pili from pathogenic Escherichia coli of turkeys.

K. V. Nagaraja, D. A. Emery, J. A. Newman, B. S. Pomeroy

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Abstract

Transmission electron microscopy was performed to identify the presence of somatic pili on Escherichia coli pathogenic to turkeys. Three common pathogenic serotypes of E coli (Ola, O2a, and O78) were used. They were cultured in tryptic soy, veal infusion, and Minca medium broth and were incubated at 37 C with and without CO2 (5%). The cultures were examined for the expression of pili after 24, 48, and 72 hours of incubation. Pili were on all strains of the 3 serotypes examined. These pili were also isolated and purified.

Original languageEnglish (US)
Pages (from-to)284-287
Number of pages4
JournalAmerican Journal of Veterinary Research
Volume44
Issue number2
StatePublished - Feb 1 1983

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