TY - JOUR
T1 - Identification of a human RAD52 pseudogene located on chromosome 2
AU - Johnson, Brad L.
AU - Campbell, Colin R
PY - 1996/3/9
Y1 - 1996/3/9
N2 - A human testis cDNA library was screened with a hybridization probe encoding the mouse RAD52 gene. Two classes of clones were identified, one derived from the human RAD52 homolog (hRAD52), the other derived from a pseudogene. In addition to many point mutations, several of which encode stop codons, the pseudogene contains a number of frame shifts and a 103-bp deletion. We further determined that the pseudogene is processed and is located on human chromosome 2, in contrast to hRAD52 which is found on chromosome 12. Reverse transcription-PCR analysis of cultured human diploid fibroblasts, as well as fibrosarcoma cells, revealed that while hRAD52 is expressed at low, but detectable levels in these cells, the pseudogene is not.
AB - A human testis cDNA library was screened with a hybridization probe encoding the mouse RAD52 gene. Two classes of clones were identified, one derived from the human RAD52 homolog (hRAD52), the other derived from a pseudogene. In addition to many point mutations, several of which encode stop codons, the pseudogene contains a number of frame shifts and a 103-bp deletion. We further determined that the pseudogene is processed and is located on human chromosome 2, in contrast to hRAD52 which is found on chromosome 12. Reverse transcription-PCR analysis of cultured human diploid fibroblasts, as well as fibrosarcoma cells, revealed that while hRAD52 is expressed at low, but detectable levels in these cells, the pseudogene is not.
KW - DNA repair
KW - Homologous recombination
KW - Molecular genetics
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U2 - 10.1016/0378-1119(95)00723-7
DO - 10.1016/0378-1119(95)00723-7
M3 - Article
C2 - 8647452
AN - SCOPUS:0029879670
SN - 0378-1119
VL - 169
SP - 229
EP - 232
JO - Gene
JF - Gene
IS - 2
ER -