Identification of a novel nonsense mutation on the Pax3 gene in ENU-derived white belly spotting mice and its genetic interaction with c-Kit

Xiao Li Guo, Hai Bin Ruan, Yan Li, Xiang Gao, Wei Li

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

In the course of a large-scale screening program of N-ethyl-N-nitrosourea mutagenesis, we isolated two semidominant mutation lines with white belly spotting, named as wps and wbs. Direct sequencing detected a nucleotide G-to-A transversion in exon 2 of the c-Kit gene in wps, which resulted in a missense D60N mutation. Another mutant, wbs, was mapped to chromosome 1 by genome-wide linkage analysis. In 93 meioses, the wbs locus was confined to a 5.2-Mb region between D1Mit380 and D1Mit215, including the Pax3 gene. A nonsense mutation K107X on the Pax3 coding region in wbs mice was identified, causing the loss of Pax3 protein in the homozygous mutant. We further demonstrated that Pax3 exhibited genetic interaction with c-Kit by intercrossing the wps and wbs mice. Further, Pax3 transactivated the c-Kit promoter in different cell lines. However, electrophoretic mobility shift assays showed that Pax3 did not bind to the c-Kit promoter, indicating that Pax3 may interact with c-Kit in an indirect way. This expands our understanding of the intricate regulatory network governing the melanocyte development.

Original languageEnglish (US)
Pages (from-to)252-262
Number of pages11
JournalPigment Cell and Melanoma Research
Volume23
Issue number2
DOIs
StatePublished - Apr 2010

Keywords

  • Genetic interaction
  • Melanocytes
  • N-ethyl-N-nitrosourea mutagenesis
  • Pax3
  • Positional cloning
  • c-Kit

Fingerprint

Dive into the research topics of 'Identification of a novel nonsense mutation on the Pax3 gene in ENU-derived white belly spotting mice and its genetic interaction with c-Kit'. Together they form a unique fingerprint.

Cite this