Identification of a sporozoite-specific antigen from toxoplasma gondii

Dolores Hill, Cathleen Coss, J. P. Dubey, Kristen Wroblewski, Mari Sautter, Tiffany Hosten, Claudia Muñoz-Zanzi, Ernest Mui, Shawn Withers, Kenneth Boyer, Gretchen Hermes, Jessica Coyne, Frank Jagdis, Andrew Burnett, Patrick McLeod, Holmes Morton, Donna Robinson, Rima McLeod

Research output: Contribution to journalArticlepeer-review

86 Scopus citations

Abstract

Reduction of risk for human and food animal infection with Toxoplasma gondii is hampered by the lack of epidemiological data documenting the predominant routes of infection (oocyst vs. tissue cyst consumption) in horizontally transmitted toxoplasmosis. Existing serological assays can determine previous exposure to the parasite, but not the route of infection. We have used difference gel electrophoresis, in combination with tandem mass spectroscopy and Western blot, to identify a sporozoite-specific protein (T. gondii embryogenesis-related protein [TgERP]), which elicited antibody and differentiated oocyst- versus tissue cyst-induced infection in pigs and mice. The recombinant protein was selected from a cDNA library constructed from T. gondii sporozoites; this protein was used in Western blots and probed with sera from T. gondii-infected humans. Serum antibody to TgERP was detected in humans within 6-8mo of initial oocyst-acquired infection. Of 163 individuals in the acute stage of infection (anti-T. gondii IgM detected in sera, or <30 in the IgG avidity test), 103 (63.2%) had detectable antibodies that reacted with TgERP. Of 176 individuals with unknown infection route and in the chronic stage of infection (no anti-T. gondii IgM detected in sera, or >30 in the IgG avidity test), antibody to TgERP was detected in 31 (17.6%). None of the 132 uninfected individuals tested had detectable antibody to TgERP. These data suggest that TgERP may be useful in detecting exposure to sporozoites in early T. gondii infection and implicates oocysts as the agent of infection.

Original languageEnglish (US)
Pages (from-to)328-337
Number of pages10
JournalJournal of Parasitology
Volume97
Issue number2
DOIs
StatePublished - Apr 2011

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