Identification of aberrantly expressed proteins in colorectal carcinoma

Ge Zhou; Yingxin Zhao; Yi Gong; Yue Chen; Carlos Becerra; Richard Gaynor; Yingming Zhao

Identification of aberrantly expressed proteins in colorectal carcinoma

Ge Zhou, Yingxin Zhao, Yi Gong, Yue Chen, Carlos Becerra, Richard Gaynor, Yingming Zhao

Biochemistry, Molecular Biology, and Biophysics (CBS)

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A proteomics approach was proposed for the profiling of protein expression in normal and colorectal cancer cells, and identification of cancer specific protein markers. Colorectal cancer specific protein markers were identified using 2D differential in-gel electrophoresis (DIGE) in combination with laser capture microdissection (LCM) and mass spectrometry. Protein expression was quantitated by the fluorescence of labelled protein in gel images. More than 20 proteins were identified in colorectal carcinoma.

Original language	English (US)
Title of host publication	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages	233-234
Number of pages	2
State	Published - Dec 1 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

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Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

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abstract = "A proteomics approach was proposed for the profiling of protein expression in normal and colorectal cancer cells, and identification of cancer specific protein markers. Colorectal cancer specific protein markers were identified using 2D differential in-gel electrophoresis (DIGE) in combination with laser capture microdissection (LCM) and mass spectrometry. Protein expression was quantitated by the fluorescence of labelled protein in gel images. More than 20 proteins were identified in colorectal carcinoma.",

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AU - Zhou, Ge

AU - Zhao, Yingxin

AU - Gong, Yi

AU - Chen, Yue

AU - Becerra, Carlos

AU - Gaynor, Richard

AU - Zhao, Yingming

PY - 2002/12/1

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AB - A proteomics approach was proposed for the profiling of protein expression in normal and colorectal cancer cells, and identification of cancer specific protein markers. Colorectal cancer specific protein markers were identified using 2D differential in-gel electrophoresis (DIGE) in combination with laser capture microdissection (LCM) and mass spectrometry. Protein expression was quantitated by the fluorescence of labelled protein in gel images. More than 20 proteins were identified in colorectal carcinoma.

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M3 - Conference contribution

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ER -

Identification of aberrantly expressed proteins in colorectal carcinoma

Abstract

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