Identification of antibodies in anti-CNS and anti-PNS myelin sera by immunoblot, characterization by immunohistochemistry, and their effect in tissue culture

Harish C. Agrawal, H. Brent Clark, Daya Agrawal, Fredrick J. Seil, Richard H. Quarles

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Immunoblot analysis of antiserum to rat central nervous system (CNS) myelin revealed antibodies to myelin basic protein (MBP), proteolipid protein (PLP), and numerous high molecular weight proteins. In addition, anti-CNS myelin serum exclusively immunostained 4 basic proteins of rat peripheral neuvous system (PNS) myelin. Similarly, anti-PNS myelin sera immunostained many high molecular weight proteins in both CNS and PNS myelin in addition to P0 and 4 basic proteins. Purified MBP and PLP were immunostained by anti-CNS myelin sera and MBP and P0 by anti-PNS myelin sera, indicating that antigenic sites are preserved during protein purification. Immunohistochemical localization with antisera was confined to the myelin sheath except that antisera to CNS myelin also stained oligodendrocytes during the active period of myelination. While anti-CNS myelin sera specifically demyelinated centrally myelinated fibers in culture, none of the anti-PNS myelin sera used here demyelinated organotypic spinal cord-dorsal root ganglion cultures.

Original languageEnglish (US)
Pages (from-to)191-200
Number of pages10
JournalBrain Research
Volume307
Issue number1-2
DOIs
StatePublished - Jul 30 1984
Externally publishedYes

Keywords

  • central nervous system
  • demyelinating antibodies
  • immunoblot technique
  • immunohistochemistry
  • myelin proteins
  • nerve tissue cultures
  • peripheral nervous system

Fingerprint

Dive into the research topics of 'Identification of antibodies in anti-CNS and anti-PNS myelin sera by immunoblot, characterization by immunohistochemistry, and their effect in tissue culture'. Together they form a unique fingerprint.

Cite this