Identification of antigenic Schistosoma mansoni glycoproteins during the course of infection in mice and humans

A P Norden, M Strand

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


The progression of humoral immune responses exhibited by mice during the year following exposure to Schistosoma mansoni cercariae was established by studying radioimmunoprecipitations of adult male and cercarial glycoproteins. 35S-methionine metabolically-labeled adult S. mansoni male worm glycoproteins precipitated by sera of 14 mice were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The initial specific antibody response (week 5) was directed against 3 worm glycoproteins of 55,000, 52,000 and 35,000 molecular weight (Mr). As infection progressed, all major worm glycoproteins (ranging from 400,000 to 12,000 Mr) were precipitated by sera from each mouse and only minor individual variations in titer were noted in the antibody responses of the mice against these glycoproteins. Maximal immunoreactivity toward the radiolabeled glycoproteins occurred at week 20 and remained at this level through week 50. Analogous experiments with sera from acutely- and chronically-infected humans resulted in immunoprecipitation patterns almost identical to those obtained with sera from the corresponding experimentally-infected mice. The kinetics of the antibody response against 125Iodine-labeled cercarial glycoproteins was the same as that observed with worm glycoproteins.

Original languageEnglish (US)
Pages (from-to)495-507
Number of pages13
JournalThe American journal of tropical medicine and hygiene
Issue number3
StatePublished - May 1985


  • Animals
  • Antibodies/immunology
  • Antibody Formation
  • Antigens, Helminth/immunology
  • Electrophoresis, Polyacrylamide Gel
  • Fasciola/immunology
  • Fascioliasis/immunology
  • Female
  • Glycoproteins/immunology
  • Humans
  • Immune Sera/immunology
  • Male
  • Mice
  • Molecular Weight
  • Rabbits/immunology
  • Schistosoma mansoni/immunology
  • Schistosomiasis/immunology
  • Sheep/immunology

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

Fingerprint Dive into the research topics of 'Identification of antigenic Schistosoma mansoni glycoproteins during the course of infection in mice and humans'. Together they form a unique fingerprint.

Cite this