Identification of N-(Deoxyguanosin-8-yl)-2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine as the Major Adduct Formed by the Food-Borne Carcinogen, 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine, with DNA

Dongxin Lin, Keith R. Kaderlik, Robert J. Turesky, Dwight W. Miller, Jackson O. Lay, Fred F. Kadlubar

Research output: Contribution to journalArticlepeer-review

185 Scopus citations

Abstract

The covalent binding of the N-acetoxy-, N-hydroxy-, and nitro derivatives of the food-borne carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) to 2′-deoxyribonucleosides or DNA was investigated in vitro and in vivo. N-Acetoxy-PhIP reacted with deoxyguanosine (dG), but not with the other deoxyribonucleosides, to form N-(deoxyguanosin-8-yl)-PhIP (dGC8- PhIP), whose structure was determined by NMR and mass spectral analyses and by ultraviolet absorption and pH-solvent partitioning characteristics. While reaction of N-acetoxy-PhIP with calf thymus DNA at pH 5.0 yielded 5.38 ± 1.16 nmol of bound PhIP residues/mg of DNA, N-hydroxy-PhIP gave only 0.13-0.23 nmol binding/mg of DNA under identical reaction conditions. Nitro-PhIP produced no detectable binding under these conditions. HPLC analysis of 1-butanol extracts of enzymatically hydrolyzed DNA that had been modified by N-acetoxy-PhIP in vitro showed a major adduct which coeluted with and had an ultraviolet absorption and a mass spectrum that were identical to that of authentic dG-C8-PhIP. 32P-Postlabeling analysis of DNA isolated from colon, pancreas, lung, heart, and liver of rats treated orally with PhIP revealed the presence of a major PhIP-DNA adduct. This adduct had chromatographic properties identical to that of the 32P-labeled bis(phosphate) derivative of dG-C8-PhIP and represented 35-45% of the total adducts. Thus, these results indicate that PhIP is similar to other carcinogenic aromatic amines,forming dG-C8-PhIP as a major DNA adduct in vitro by reaction of N-acetoxy-PhIP with DNA and also in vivo in a variety of rat tissues after oral dosing with PhIP.

Original languageEnglish (US)
Pages (from-to)691-697
Number of pages7
JournalChemical research in toxicology
Volume5
Issue number5
DOIs
StatePublished - Sep 1 1992

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