TY - JOUR
T1 - Identification of RAPD markers linked to common bacterial blight resistance genes in Phaseolus vulgaris L.
AU - Bai, Yonghe
AU - Michaels, T. E.
AU - Pauls, K. P.
PY - 1997
Y1 - 1997
N2 - Seven hundred and fifty-six random primers were screened with bulks of genomic DNA from common bacterial blight (CBB) resistant and susceptible bean plants. The plants were from a breeding population derived from an interspecific cross between Phaseolus acutifolius and Phaseolus vulgaris. Four RAPD markers, named R7313, RE416, RE49, and R4865, were found to be significantly associated with CBB resistance in this population. Forty-nine molecular markers segregating in the population were clustered into 8 linkage groups by a MAPMAKER linkage analysis. The largest linkage group was 140 cM long and contained 25 marker loci, including marker R4865. Markers R7313, RE416, and RE49 were clustered on another linkage group. A regression analysis indicated that the markers in these two groups together accounted for 81% of the variation in CBB resistance in the population. The addition of another marker, M56810, which was not individually associated with CBB resistance, increased the total contribution to the trait to 87%.
AB - Seven hundred and fifty-six random primers were screened with bulks of genomic DNA from common bacterial blight (CBB) resistant and susceptible bean plants. The plants were from a breeding population derived from an interspecific cross between Phaseolus acutifolius and Phaseolus vulgaris. Four RAPD markers, named R7313, RE416, RE49, and R4865, were found to be significantly associated with CBB resistance in this population. Forty-nine molecular markers segregating in the population were clustered into 8 linkage groups by a MAPMAKER linkage analysis. The largest linkage group was 140 cM long and contained 25 marker loci, including marker R4865. Markers R7313, RE416, and RE49 were clustered on another linkage group. A regression analysis indicated that the markers in these two groups together accounted for 81% of the variation in CBB resistance in the population. The addition of another marker, M56810, which was not individually associated with CBB resistance, increased the total contribution to the trait to 87%.
KW - Common bacterial blight (CBB)
KW - Linkage groups
KW - Phaseolus vulgaris L.
KW - Polymerase chain reaction (PCR)
KW - RAPD markers
UR - http://www.scopus.com/inward/record.url?scp=0031427893&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031427893&partnerID=8YFLogxK
U2 - 10.1139/g97-071
DO - 10.1139/g97-071
M3 - Article
C2 - 18464845
AN - SCOPUS:0031427893
SN - 0831-2796
VL - 40
SP - 544
EP - 551
JO - Genome
JF - Genome
IS - 4
ER -