Identification of the amino acid-AZT-phosphoramidase by affinity T7 phage display selection

Jilin Cheng, Xin Zhou, Tsui Fen Chou, Brahma Ghosh, Baoling Liu, Carston R. Wagner

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

A CEM cell cDNA T7 phage display library was prepared and used to screen for activating enzymes of phosphoramidate prodrugs of AZT monophosphate. Although, inefficient compared to ribonucleotide based phosphoramidates, hHint 1 was identified as the likely intracellular pronucleotide activating enzyme.

Original languageEnglish (US)
Pages (from-to)6379-6381
Number of pages3
JournalBioorganic and Medicinal Chemistry Letters
Volume19
Issue number22
DOIs
StatePublished - 2009

Bibliographical note

Funding Information:
This work was partially supported by Grant CA-61909 and the University of Minnesota Graduate School (C.R.W).

Keywords

  • AZT
  • Hint1
  • Phosphoramidate
  • Prodrug
  • Pronucleotide

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