IL-13 enhances agonist-evoked calcium signals and contractile responses in airway smooth muscle

Omar Tliba, Deepak Deshpande, Hang Chen, Christopher Van Besien, Mathur S Kannan, Reynold A. Panettieri, Yassine Amrani

Research output: Contribution to journalArticlepeer-review

135 Scopus citations

Abstract

Growing evidence suggests that interleukin (IL)-13, a Th2-type cytokine, plays a critical role in the development of bronchial hyper-responsiveness (BHR), an essential feature of asthma, although the underlying mechanisms remain unknown. In the present study, we investigated whether IL-13 directly affects airway smooth muscle (ASM) function. In murine tracheal rings, IL-13 (100 ng ml -1, 24 h) significantly increased both the carbachol- and KCl-induced maximal force generation without affecting ASM sensitivity. In cultured human ASM cells, IL-13 (50 ng ml -1, 24 h) also augmented cytosolic calcium levels to bradykinin, histamine and carbachol by 60, 35 and 26%, respectively. The present study demonstrates that IL-13 may promote BHR by directly modulating ASM contractility, an effect that may be due to enhanced G protein-coupled receptor (GPCR)-associated calcium signaling.

Original languageEnglish (US)
Pages (from-to)1159-1162
Number of pages4
JournalBritish Journal of Pharmacology
Volume140
Issue number7
DOIs
StatePublished - Dec 2003

Keywords

  • Airway smooth muscle
  • Asthma
  • Calcium metabolism
  • Isometric tension
  • Th2 cytokine

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