Imaging arterial cells, atherosclerosis, and restenosis by multimodal nonlinear optical microscopy

Han Wei Wang, Vlad Simianu, Mattew J. Locker, Michael Sturek, Ji Xin Cheng

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations


By integrating sum-frequency generation (SFG), and two-photon excitation fluorescence (TPEF) on a coherent antiStokes Raman scattering (CARS) microscope platform, multimodal nonlinear optical (NLO) imaging of arteries and atherosclerotic lesions was demonstrated. CARS signals arising from CH 2-rich membranes allowed visualization of endothelial cells and smooth muscle cells in a carotid artery. Additionally, CARS microscopy allowed vibrational imaging of elastin and collagen fibrils which are rich in CH 2 bonds in their cross-linking residues. The extracellular matrix organization was further confirmed by TPEF signals arising from elastin's autofluorescence and SFG signals arising from collagen fibrils' non-centrosymmetric structure. The system is capable of identifying different atherosclerotic lesion stages with sub-cellular resolution. The stages of atherosclerosis, such as macrophage infiltration, lipid-laden foam cell accumulation, extracellular lipid distribution, fibrous tissue deposition, plaque establishment, and formation of other complicated lesions could be viewed by our multimodal CARS microscope. Collagen percentages in the region adjacent to coronary artery stents were resolved. High correlation between NLO and histology imaging evidenced the validity of the NLO imaging. The capability of imaging significant components of an arterial wall and distinctive stages of atherosclerosis in a label-free manner suggests the potential application of multimodal nonlinear optical microscopy to monitor the onset and progression of arterial diseases.

Original languageEnglish (US)
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences VIII
StatePublished - Apr 23 2008
EventMultiphoton Microscopy in the Biomedical Sciences VIII - San Jose, CA, United States
Duration: Jan 20 2008Jan 22 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherMultiphoton Microscopy in the Biomedical Sciences VIII
CountryUnited States
CitySan Jose, CA


  • Atherosclerosis
  • CARS
  • Collagen
  • Foam cell
  • Multimodal


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