Immobilization of histidine-tagged proteins by magnetic nanoparticles encapsulated with nitrilotriacetic acid (NTA)-phospholipids micelle

Yong Taik Lim; Kun Yeong Lee; Kwangyeol Lee; Bong Hyun Chung

doi:10.1016/j.bbrc.2006.03.209

Immobilization of histidine-tagged proteins by magnetic nanoparticles encapsulated with nitrilotriacetic acid (NTA)-phospholipids micelle

Yong Taik Lim, Kun Yeong Lee, Kwangyeol Lee, Bong Hyun Chung

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

We described the development of functionalized magnetic nanoparticles (MNPs) with PEG-modification, a phospholipids micelle coating, and their use in manipulating histidine-tagged proteins. Highly monodisperse MNPs were synthesized in an organic solvent and could be phase-transferred into an aqueous solution by encapsulating the nanoparticles with a phospholipids micelle. The phospholipids micelle coating rendered the nanoparticles highly water-soluble, and the functional groups of the phospholipids coating allowed for the bioconjugation of various moieties, such as fluorescent molecules and engineered proteins. Functionalized phospholipids, such as nitrilotriacetic acid (NTA)-phospholipids, caused the MNPs to bind and allowed for manipulation of histidine-tagged proteins. Due to their high surface/volume ratio, the MNPs showed better performance (about 100 times higher) in immobilizing engineered proteins than conventional micrometer-sized beads. This demonstrates that MNPs coated with phospholipids micelle can be a versatile platform for the effective manipulation of various kinds of engineered proteins, which is very important in the field of proteomics. It is expected that a combination of MNPs with optical fluorescent molecules can find applications in bimodal (magnetic and optical) molecular imaging nanoprobes.

Original language	English (US)
Pages (from-to)	926-930
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	344
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2006.03.209
State	Published - Jun 9 2006
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by Grant No. RTI04-03-06 from the Regional Technology Innovation Program of the Ministry of Commerce, Industry and Energy (MOCIE), the KETI Research Program (Grant No. 10023796-2005-01), and the KRIBB Research Initiative Program.

Keywords

Fluorescent proteins
Immobilization of proteins
Magnetic nanoparticles
NTA-phospholipids
Phospholipids micelle

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title = "Immobilization of histidine-tagged proteins by magnetic nanoparticles encapsulated with nitrilotriacetic acid (NTA)-phospholipids micelle",

abstract = "We described the development of functionalized magnetic nanoparticles (MNPs) with PEG-modification, a phospholipids micelle coating, and their use in manipulating histidine-tagged proteins. Highly monodisperse MNPs were synthesized in an organic solvent and could be phase-transferred into an aqueous solution by encapsulating the nanoparticles with a phospholipids micelle. The phospholipids micelle coating rendered the nanoparticles highly water-soluble, and the functional groups of the phospholipids coating allowed for the bioconjugation of various moieties, such as fluorescent molecules and engineered proteins. Functionalized phospholipids, such as nitrilotriacetic acid (NTA)-phospholipids, caused the MNPs to bind and allowed for manipulation of histidine-tagged proteins. Due to their high surface/volume ratio, the MNPs showed better performance (about 100 times higher) in immobilizing engineered proteins than conventional micrometer-sized beads. This demonstrates that MNPs coated with phospholipids micelle can be a versatile platform for the effective manipulation of various kinds of engineered proteins, which is very important in the field of proteomics. It is expected that a combination of MNPs with optical fluorescent molecules can find applications in bimodal (magnetic and optical) molecular imaging nanoprobes.",

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note = "Funding Information: This work was supported by Grant No. RTI04-03-06 from the Regional Technology Innovation Program of the Ministry of Commerce, Industry and Energy (MOCIE), the KETI Research Program (Grant No. 10023796-2005-01), and the KRIBB Research Initiative Program.",

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Immobilization of histidine-tagged proteins by magnetic nanoparticles encapsulated with nitrilotriacetic acid (NTA)-phospholipids micelle

Abstract

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Keywords

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