Immunohistochemical colocalization of GABA and insulin in β-cells of rat islet

D. J. Garry; R. L. Sorenson; R. P. Elde; B. E. Maley; A. Madsen

doi:10.2337/diab.35.10.1090

Immunohistochemical colocalization of GABA and insulin in β-cells of rat islet

D. J. Garry, R. L. Sorenson, R. P. Elde, B. E. Maley, A. Madsen

Medicine - Cardiology Division

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Abstract

γ-Aminobutyric acid (GABA) is found in high concentrations in the pancreatic islet. In addition, enzymes regulating the level of GABA (L-glutamated decarboxylase and GABA-α-ketoglutarate transaminase) have been immunohistochemically localized in the medullary cells of the islet. In this study, an immunofluorescence and elution/restaining protocol is used to determine the distribution of GABA and either insulin, glucagon, or somatostatin in a tissue section. GABA was not detected within the iselt α- or δ-cells but was determined to be localized within the insulin-containing β-cells.

Original language	English (US)
Pages (from-to)	1090-1095
Number of pages	6
Journal	Diabetes
Volume	35
Issue number	10
DOIs	https://doi.org/10.2337/diab.35.10.1090
State	Published - 1986

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AU - Madsen, A.

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AB - γ-Aminobutyric acid (GABA) is found in high concentrations in the pancreatic islet. In addition, enzymes regulating the level of GABA (L-glutamated decarboxylase and GABA-α-ketoglutarate transaminase) have been immunohistochemically localized in the medullary cells of the islet. In this study, an immunofluorescence and elution/restaining protocol is used to determine the distribution of GABA and either insulin, glucagon, or somatostatin in a tissue section. GABA was not detected within the iselt α- or δ-cells but was determined to be localized within the insulin-containing β-cells.

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Immunohistochemical colocalization of GABA and insulin in β-cells of rat islet

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