Immunohistochemical colocalization of GABA and insulin in β-cells of rat islet

D. J. Garry, R. L. Sorenson, R. P. Elde, B. E. Maley, A. Madsen

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

γ-Aminobutyric acid (GABA) is found in high concentrations in the pancreatic islet. In addition, enzymes regulating the level of GABA (L-glutamated decarboxylase and GABA-α-ketoglutarate transaminase) have been immunohistochemically localized in the medullary cells of the islet. In this study, an immunofluorescence and elution/restaining protocol is used to determine the distribution of GABA and either insulin, glucagon, or somatostatin in a tissue section. GABA was not detected within the iselt α- or δ-cells but was determined to be localized within the insulin-containing β-cells.

Original languageEnglish (US)
Pages (from-to)1090-1095
Number of pages6
JournalDiabetes
Volume35
Issue number10
DOIs
StatePublished - Jan 1 1986

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