In the immunohistochemical analysis of the brain, tissue preparation and fixation are critical steps. During our studies of transplanting human bone marrow multipotent adult progenitor cells (hMAPCs) into the rat brain, we noticed that various methods of brain tissue preparation and fixation differentially influenced antigenic preservation in the transplants and in the host brain. Here, we report a simple, effective and reproducible method of tissue preparation and fixation that results in the immunohistochemical labeling of transplanted and host cells.
Bibliographical noteFunding Information:
The authors wish to thank William F. Kaemmerer and Michael McPhee for help with the graphics, Dirk Keene for expanding hMAPCs, Zhenhong Nan for sectioning the neonatal brain and Kirstin Olmon for her administrative assistance. This work was supported by the Lyle French Neurosurgery Research Fund, the Ben and Beryl Miller Fund, the Cornford Endowment Fund, Supporters’ United for Parkinson’s Education and Research (SUPER) Fund, the Dorothy Cavine Endowment, the Elmer Isakson Parkinson’s Disease Fund, the Children’s Cancer Research Fund, University of Minnesota Bone Marrow Transplant Research Fund, the Leukemia Society of America 1F31AI/GM10291 and NIH grants 1F30MH12157, RO1-HL-69137, and RO1-NS40831.
- Bone marrow stem cell
- Fast frozen