Immunohistochemical Localization of Insulin-Degrading Enzyme along the Rat Intestine, in the Human Colon Adenocarcinoma Cell Line (Caco-2), and in Human Ileum

Li Ling Chang; Laurence E. Stout; W. Douglas Wong; John G. Buls; David A. Rothenberger; W. Thomas Shier; Robert L. Sorenson; Jane P.F. Bai

doi:10.1021/js960035q

Immunohistochemical Localization of Insulin-Degrading Enzyme along the Rat Intestine, in the Human Colon Adenocarcinoma Cell Line (Caco-2), and in Human Ileum

Li Ling Chang, Laurence E. Stout, W. Douglas Wong, John G. Buls, David A. Rothenberger, W. Thomas Shier, Robert L. Sorenson, Jane P.F. Bai

Research output: Contribution to journal › Article › peer-review

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Abstract

Insulin-degrading enzyme (IDE) has been implicated in the intracellular degradation of insulin in insulin target cells. Knowledge of the existence of this enzyme in the intestine will be beneficial to the achievement of clinical oral efficacy of insulin. A comparative study was conducted with rat intestine, human colon adenocarcinoma (Caco-2) cells, and human ileum. Confocal microscopy analysis using the anti-IDE antibody showed that IDE was localized in the mucosal cells of rat and human intestines, as well as in Caco-2 cells. Immunostaining of this enzyme was homogeneous throughout the cell excluding nucleus, indicating a typical cytosolic distribution in rat and human enterocytes and in Caco-2 cells.

Original language	English (US)
Pages (from-to)	116-119
Number of pages	4
Journal	Journal of Pharmaceutical Sciences
Volume	86
Issue number	1
DOIs	https://doi.org/10.1021/js960035q
State	Published - 1997

Bibliographical note

Funding Information:
The authors are grateful to Ms. Celest M. Roth for her excellent technical assistance and to Ms. Carol M. Bigalke for her kind assistance. Partial financial support from AAPS Young Investigator Award is acknowledged.

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Chang, L. L., Stout, L. E., Wong, W. D., Buls, J. G., Rothenberger, D. A., Shier, W. T., Sorenson, R. L., & Bai, J. P. F. (1997). Immunohistochemical Localization of Insulin-Degrading Enzyme along the Rat Intestine, in the Human Colon Adenocarcinoma Cell Line (Caco-2), and in Human Ileum. Journal of Pharmaceutical Sciences, 86(1), 116-119. https://doi.org/10.1021/js960035q

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title = "Immunohistochemical Localization of Insulin-Degrading Enzyme along the Rat Intestine, in the Human Colon Adenocarcinoma Cell Line (Caco-2), and in Human Ileum",

abstract = "Insulin-degrading enzyme (IDE) has been implicated in the intracellular degradation of insulin in insulin target cells. Knowledge of the existence of this enzyme in the intestine will be beneficial to the achievement of clinical oral efficacy of insulin. A comparative study was conducted with rat intestine, human colon adenocarcinoma (Caco-2) cells, and human ileum. Confocal microscopy analysis using the anti-IDE antibody showed that IDE was localized in the mucosal cells of rat and human intestines, as well as in Caco-2 cells. Immunostaining of this enzyme was homogeneous throughout the cell excluding nucleus, indicating a typical cytosolic distribution in rat and human enterocytes and in Caco-2 cells.",

author = "Chang, {Li Ling} and Stout, {Laurence E.} and Wong, {W. Douglas} and Buls, {John G.} and Rothenberger, {David A.} and Shier, {W. Thomas} and Sorenson, {Robert L.} and Bai, {Jane P.F.}",

note = "Funding Information: The authors are grateful to Ms. Celest M. Roth for her excellent technical assistance and to Ms. Carol M. Bigalke for her kind assistance. Partial financial support from AAPS Young Investigator Award is acknowledged.",

year = "1997",

doi = "10.1021/js960035q",

language = "English (US)",

volume = "86",

pages = "116--119",

journal = "Journal of Pharmaceutical Sciences",

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AU - Chang, Li Ling

AU - Stout, Laurence E.

AU - Wong, W. Douglas

AU - Buls, John G.

AU - Rothenberger, David A.

AU - Shier, W. Thomas

AU - Sorenson, Robert L.

AU - Bai, Jane P.F.

N1 - Funding Information: The authors are grateful to Ms. Celest M. Roth for her excellent technical assistance and to Ms. Carol M. Bigalke for her kind assistance. Partial financial support from AAPS Young Investigator Award is acknowledged.

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N2 - Insulin-degrading enzyme (IDE) has been implicated in the intracellular degradation of insulin in insulin target cells. Knowledge of the existence of this enzyme in the intestine will be beneficial to the achievement of clinical oral efficacy of insulin. A comparative study was conducted with rat intestine, human colon adenocarcinoma (Caco-2) cells, and human ileum. Confocal microscopy analysis using the anti-IDE antibody showed that IDE was localized in the mucosal cells of rat and human intestines, as well as in Caco-2 cells. Immunostaining of this enzyme was homogeneous throughout the cell excluding nucleus, indicating a typical cytosolic distribution in rat and human enterocytes and in Caco-2 cells.

AB - Insulin-degrading enzyme (IDE) has been implicated in the intracellular degradation of insulin in insulin target cells. Knowledge of the existence of this enzyme in the intestine will be beneficial to the achievement of clinical oral efficacy of insulin. A comparative study was conducted with rat intestine, human colon adenocarcinoma (Caco-2) cells, and human ileum. Confocal microscopy analysis using the anti-IDE antibody showed that IDE was localized in the mucosal cells of rat and human intestines, as well as in Caco-2 cells. Immunostaining of this enzyme was homogeneous throughout the cell excluding nucleus, indicating a typical cytosolic distribution in rat and human enterocytes and in Caco-2 cells.

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Immunohistochemical Localization of Insulin-Degrading Enzyme along the Rat Intestine, in the Human Colon Adenocarcinoma Cell Line (Caco-2), and in Human Ileum

Abstract

Bibliographical note

UN SDGs

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