Immunohistochemical Localization of Pasteurella haemolytica Al-derived Endotoxin, Leukotoxin, and Capsular Polysaccharide in Experimental Bovine Pasteurella Pneumonia

L. O. Whiteley, S. K. Maheswaran, D. J. Weiss, T. R. Ames

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Abstract

Six, 5- to 10-week-old male Holstein calves were inoculated intratracheally with 5 × 10′ logarithmic growth phase Pasteurella haemolytica biotype A serotype 1 (A1). Immunohistochemical techniques in conjunction with the use of monoclonal antibodies directed against P. haemolytica A1-derived lipopolysaccharide (LPS), capsular polysaccharide, and a polyclonal rabbit anti-leukotoxin antibody were used to localize their respective antigens in tissue sections of pneumonic lung at the light and electron microscopic levels. We found the following: 1) LPS, capsular polysaccharide, and leukotoxin were released into the inflammatory exudate; 2) LPS was found within the cytoplasm of neutrophils (located in the alveolus and alveolar wall), alveolar macrophages, endothelial cells, pulmonary intravascular macrophages, and on epithelial cell surfaces; 3) capsular polysaccharide was found in the alveolus and alveolar macrophages but not in cells of the alveolar wall; and 4) leukotoxin was associated with cell membranes of degenerating inflammatory cells located in the alveolus. This is the first study that demonstrates the presence of leukotoxin in the pulmonary inflammatory lesions caused by P. haemolytica A1 and implicates endotoxin as an important factor in the genesis of the pulmonary lesions.

Original languageEnglish (US)
Pages (from-to)150-161
Number of pages12
JournalVeterinary pathology
Volume27
Issue number3
DOIs
StatePublished - May 1990

Bibliographical note

Funding Information:
This work was submitted as part of the senior author's PhD thesis and was made possible by a special grant, 85CRSR-2-2629, from the US Department of Agriculture. We thank Dr. Evelyn L. Townsend for expert technical assistance.

Keywords

  • Bovine
  • Pasteurella haemolytica
  • endotoxin
  • immunohistochemistry
  • leukotoxin
  • lung

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