Immunohistochemistry was used to demonstrate the presence of three proteins (actin, osteocalcin and alkaline phosphatase) within the same ethylenediaminetetraacetic acid (EDTA) decalcified and paraffin embedded bone tissue section. EDTA chelated calcium ions, causing endogenous alkaline phosphatase (ALP) to be inactivated. By ablating endogenous ALP activity through chelation, we were able to use an ALP based detection system before reactivation of the endogenous enzyme with magnesium chloride. After reactivation, endogenous ALP itself was demonstrated immunohistochemically. (The J Histotechnol 15:93, 1992).
Bibliographical noteFunding Information:
The authors thank Dr Carolyn Moyer for her initial support in establishing our immunohistochemistry laboratory and Mrs. Karen Holbrook for manuscript preparation. Supported in part by NIH grant numbers RR00919 and HL14164
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- Alkaline phosphatase