The application of an immunoperoxidase method to labeling of Streptococcus mutans subsp. sobrinus for scanning electron microscopy is described. Bacteria from four separate cultures were fixed in 2.5% buffered glutaraldehyde solution. The specimens were treated in accordance with an indirect immunolabeling procedure using a rabbit anti-S. mutans subsp. sobrinus γglobulin. Labeled bacteria were equipped with a layer of small, distinct globules of horseradish peroxidase reaction product. Bacteria exposed to control incubations were not equipped with this layer.
Bibliographical noteFunding Information:
Acknowledgements.-This work was supported by a grant from the Faculty of Odontology, Karolinska Institute, Stockholm, and by USPHS grants DE-02636 and DE-07085 from the National Institute for Dental Research (NIDR). Parts of this paper were presented at the 54th General Session of the International Association for Dental Research (1976).
- Horseradish peroxidase