Immunoperoxidase labeling of streptococcus mutans for scanning electron microscopy

Peter Berthold

Research output: Contribution to journalArticlepeer-review

Abstract

The application of an immunoperoxidase method to labeling of Streptococcus mutans subsp. sobrinus for scanning electron microscopy is described. Bacteria from four separate cultures were fixed in 2.5% buffered glutaraldehyde solution. The specimens were treated in accordance with an indirect immunolabeling procedure using a rabbit anti-S. mutans subsp. sobrinus γglobulin. Labeled bacteria were equipped with a layer of small, distinct globules of horseradish peroxidase reaction product. Bacteria exposed to control incubations were not equipped with this layer.

Original languageEnglish (US)
Pages (from-to)125-128
Number of pages4
JournalActa Odontologica Scandinavica
Volume41
Issue number2
DOIs
StatePublished - 1983

Bibliographical note

Funding Information:
Acknowledgements.-This work was supported by a grant from the Faculty of Odontology, Karolinska Institute, Stockholm, and by USPHS grants DE-02636 and DE-07085 from the National Institute for Dental Research (NIDR). Parts of this paper were presented at the 54th General Session of the International Association for Dental Research (1976).

Keywords

  • Bacteria
  • Horseradish peroxidase
  • Immunocytochemistry
  • Ultrastructure

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