TY - JOUR
T1 - Immunophysiological studies of interleukin-2 and canine lymphocytes
AU - Helfand, Stuart C.
AU - Modiano, Jaime F.
AU - Nowell, Peter C.
N1 - Funding Information:
This researchw ass upporteidn part by grantN IH-CA 42232 (PCN) and a grant from the GraduateS chool of the University of Wisconsin-Madison (SCH).
PY - 1992/6
Y1 - 1992/6
N2 - Interleukin-2-dependent pathways of lymphocyte activation were investigated in canine peripheral blood lymphocytes (PBL) following stimulation with T-cell mitogens including phytohemagglutinin, phorbol ester (TPA), calcium ionophore (ionomycin), and human recombinant interleukin-2 (hrIL-2). The ability of the stimulated cells to produce interleukin-2 (IL-2) was determined using murine indicator cell lines. IL-2 receptor expression by mitogen-stimulated canine PBL was confirmed by the binding of hrlL-2 with high affinity, and with characteristics comparable to those of the human and murine IL-2 receptor. Examination of serum and PBL from two dogs that were treated with hrlL-2 and human recombinant tumor necrosis factor for systemic mast cell tumors showed that in one dog, IL-2 could be measured in the serum. Concurrently, the in vitro mitogenic response of this dog's PBL to hrlL-2 occurred earlier, possibly reflecting an increase in the relative number of IL-2-responsive cells within the PBL population.
AB - Interleukin-2-dependent pathways of lymphocyte activation were investigated in canine peripheral blood lymphocytes (PBL) following stimulation with T-cell mitogens including phytohemagglutinin, phorbol ester (TPA), calcium ionophore (ionomycin), and human recombinant interleukin-2 (hrIL-2). The ability of the stimulated cells to produce interleukin-2 (IL-2) was determined using murine indicator cell lines. IL-2 receptor expression by mitogen-stimulated canine PBL was confirmed by the binding of hrlL-2 with high affinity, and with characteristics comparable to those of the human and murine IL-2 receptor. Examination of serum and PBL from two dogs that were treated with hrlL-2 and human recombinant tumor necrosis factor for systemic mast cell tumors showed that in one dog, IL-2 could be measured in the serum. Concurrently, the in vitro mitogenic response of this dog's PBL to hrlL-2 occurred earlier, possibly reflecting an increase in the relative number of IL-2-responsive cells within the PBL population.
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U2 - 10.1016/0165-2427(92)90030-T
DO - 10.1016/0165-2427(92)90030-T
M3 - Article
C2 - 1632072
AN - SCOPUS:0026691964
SN - 0165-2427
VL - 33
SP - 1
EP - 16
JO - Veterinary immunology and immunopathology
JF - Veterinary immunology and immunopathology
IS - 1-2
ER -
