Immunoreactive peptides related to dynorphin B (=rimorphin) in the rat brain

Y. X. Zhu, V. Höllt, H. Loh

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

We have developed a radioimmunoassay for synthetic dynorphin B, a novel opioid tridecapeptide, which shares a common precursor molecule with dynorphin1-17 (=dynorphin A) and the neo-endorphins. The levels of immunoreactivity towards this peptide in rat brain and pituitary show a pattern quantitatively and qualitatively similar to those found for dynorphin A and α-neo-endorphin in earlier studies. The antiserum used was highly specific with only dynorphin-32 and dynorphin B-29, both of which contain the dynorphin B sequence, showing substantial cross-reactivity. Gel filtration of whole rat brain extracts in combination with HPLC analysis provide strong evidence for the existence of these latter two peptides in rat brain.

Original languageEnglish (US)
Pages (from-to)871-874
Number of pages4
JournalPeptides
Volume4
Issue number6
DOIs
StatePublished - Jan 1 1983

Keywords

  • Dynorphin B
  • Dynorphin B-29
  • Dynorphin-32

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