Abstract
We have developed a radioimmunoassay for synthetic dynorphin B, a novel opioid tridecapeptide, which shares a common precursor molecule with dynorphin1-17 (=dynorphin A) and the neo-endorphins. The levels of immunoreactivity towards this peptide in rat brain and pituitary show a pattern quantitatively and qualitatively similar to those found for dynorphin A and α-neo-endorphin in earlier studies. The antiserum used was highly specific with only dynorphin-32 and dynorphin B-29, both of which contain the dynorphin B sequence, showing substantial cross-reactivity. Gel filtration of whole rat brain extracts in combination with HPLC analysis provide strong evidence for the existence of these latter two peptides in rat brain.
Original language | English (US) |
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Pages (from-to) | 871-874 |
Number of pages | 4 |
Journal | Peptides |
Volume | 4 |
Issue number | 6 |
DOIs | |
State | Published - Jan 1 1983 |
Keywords
- Dynorphin B
- Dynorphin B-29
- Dynorphin-32