Immunostimulatory effects of human recombinant interleukin-12 on peripheral blood mononuclear cells from normal dogs

Brenda S. Phillips, Marcia L. Padilla, Erin B. Dickerson, Mary J. Lindstrom, Stuart C. Helfand

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Interleukin-12 (IL-12) plays a pivotal role in regulating cellular immune responses involving autoimmunity, infectious disease, and cancer. Human recombinant (hr) IL-12 is being evaluated for therapy of human cancer. We investigated the potential of hrIL-12 to activate canine peripheral blood mononuclear cells (PBMC) using proliferation and cytotoxicity as readouts. Human rIL-12 caused increased proliferation of PBMC, and enhanced lysis of allogeneic canine tumor targets mediated by PBMC from normal dogs in vitro. In addition, antibody-dependent cellular cytotoxicity (ADCC) mediated by canine PBMC was enhanced by hrIL-12. These results indicate that hrIL-12 is recognized by canine immune cells, triggering a number of immune responses in canine PBMC, that may be important for immunotherapy of canine cancer. Information from this investigation provides impetus for evaluation of the effects of hrIL-12 on PBMC from tumor-bearing dogs and should be helpful in the development of hrIL-12 as an immune cell activator in vivo in the dog.

Original languageEnglish (US)
Pages (from-to)189-201
Number of pages13
JournalVeterinary immunology and immunopathology
Volume70
Issue number3-4
DOIs
StatePublished - Sep 20 1999

Bibliographical note

Funding Information:
This work was supported by NIH grant CA01696 (SCH), Morris Animal Foundation grant 98CA-04 (SCH) and a University of Wisconsin, School of Veterinary Medicine Companion Animal Grant (BP). The authors would like to thank Dr. Paul Sondel and his laboratory for helpful suggestions and technical assistance.

Keywords

  • Antibody-dependent cellular cytotoxicity
  • Cytotoxicity
  • Dog
  • Interleukin-12
  • Peripheral blood mononuclear cells
  • Proliferation

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