Impact of inhibiting activity of indole inhibitors on phospholipid hydrolysis by phospholipase A2

Xiuhong Zhai; Gerald Brezesinski; Helmuth Möhwald; Junbai Li

doi:10.1016/j.colsurfa.2004.09.016

Impact of inhibiting activity of indole inhibitors on phospholipid hydrolysis by phospholipase A₂

Xiuhong Zhai, Gerald Brezesinski, Helmuth Möhwald, Junbai Li

Hormel Institute

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The influence of two indole inhibitors on the hydrolysis reaction of l-dipalmitoylphosphatidylcholine (l-DPPC) catalyzed by phospholipase A ₂ (PLA₂) has been studied in the coexistence region using surface pressure as function of time (π-t) and Brewster angle microscopy (BAM) measurements. Similar experiments have been performed with l-DPPC vesicles made by ultrasonication method using transmission electron microscopy (TEM). Comparing the π-t curves and BAM images, which show defected domains after a certain reaction time, as well as TEM images of defected vesicles, one can conclude that the two indole inhibitors have different abilities in reducing the activity of PLA₂. The described experiments show that BAM and TEM can be successfully used to study the influence of newly designed inhibitors on the hydrolysis reaction catalyzed by PLA₂.

Original language	English (US)
Pages (from-to)	51-55
Number of pages	5
Journal	Colloids and Surfaces A: Physicochemical and Engineering Aspects
Volume	256
Issue number	1 SPEC. ISS.
DOIs	https://doi.org/10.1016/j.colsurfa.2004.09.016
State	Published - Apr 1 2005

Bibliographical note

Funding Information:
We acknowledge the financial support of the National Nature Science Foundation of China (NNSFC 29925307), the Major State Basic Research Development Program (973, Grant No. G2000078103), the Chinese Academy of Sciences as well as the collaboration project of the German Max Planck Society.

Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.

Keywords

Catalyzed
Indole
PLA

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Impact of inhibiting activity of indole inhibitors on phospholipid hydrolysis by phospholipase A₂ . / Zhai, Xiuhong; Brezesinski, Gerald; Möhwald, Helmuth et al.
In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 256, No. 1 SPEC. ISS., 01.04.2005, p. 51-55.

Research output: Contribution to journal › Article › peer-review

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abstract = "The influence of two indole inhibitors on the hydrolysis reaction of l-dipalmitoylphosphatidylcholine (l-DPPC) catalyzed by phospholipase A 2 (PLA2) has been studied in the coexistence region using surface pressure as function of time (π-t) and Brewster angle microscopy (BAM) measurements. Similar experiments have been performed with l-DPPC vesicles made by ultrasonication method using transmission electron microscopy (TEM). Comparing the π-t curves and BAM images, which show defected domains after a certain reaction time, as well as TEM images of defected vesicles, one can conclude that the two indole inhibitors have different abilities in reducing the activity of PLA2. The described experiments show that BAM and TEM can be successfully used to study the influence of newly designed inhibitors on the hydrolysis reaction catalyzed by PLA2.",

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