The influence of two indole inhibitors on the hydrolysis reaction of l-dipalmitoylphosphatidylcholine (l-DPPC) catalyzed by phospholipase A 2 (PLA2) has been studied in the coexistence region using surface pressure as function of time (π-t) and Brewster angle microscopy (BAM) measurements. Similar experiments have been performed with l-DPPC vesicles made by ultrasonication method using transmission electron microscopy (TEM). Comparing the π-t curves and BAM images, which show defected domains after a certain reaction time, as well as TEM images of defected vesicles, one can conclude that the two indole inhibitors have different abilities in reducing the activity of PLA2. The described experiments show that BAM and TEM can be successfully used to study the influence of newly designed inhibitors on the hydrolysis reaction catalyzed by PLA2.
|Original language||English (US)|
|Number of pages||5|
|Journal||Colloids and Surfaces A: Physicochemical and Engineering Aspects|
|Issue number||1 SPEC. ISS.|
|State||Published - Apr 1 2005|
Bibliographical noteFunding Information:
We acknowledge the financial support of the National Nature Science Foundation of China (NNSFC 29925307), the Major State Basic Research Development Program (973, Grant No. G2000078103), the Chinese Academy of Sciences as well as the collaboration project of the German Max Planck Society.
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