Improved cellulase production via disruption of PDE01641 in cellulolytic fungus Penicillium decumbens

Yanmei Zhang; Jiwei Zhang; Peng Xiao; Tianhong Wang; Yinbo Qu

doi:10.1016/j.biortech.2012.07.101

Improved cellulase production via disruption of PDE01641 in cellulolytic fungus Penicillium decumbens

Yanmei Zhang, Jiwei Zhang, Peng Xiao, Tianhong Wang, Yinbo Qu

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

To investigate the function of PDE01641, the homologue of Neurospora crassa NCU05137, during cellulase production in Penicillium decumbens, the PDE01641 encoding gene was knocked out in the present work. The PDE01641 deletion mutant showed enhanced cellobiohydrolase activity and β-glucosidase activity compared to the parental strain 114-2. Increased transcription of the main cellulase and hemicellulase genes in Δ. PDE01641 gave evidence that PDE01641 might affect the process associated with the regulation of cellulolytic enzymes expression. Furthermore, the deletion of PDE01641 from the genome of hypercellulolytic industrial strain JU-A10-T resulted in 36% and 80% increase in cellulase activity and hemicellulase activity respectively. These results revealed that PDE01641 plays an important role in the regulation of cellulolytic enzyme production in P. decumbens, and the engineering strain constructed in this work could be potentially used in bioenergy production.

Original language	English (US)
Pages (from-to)	733-737
Number of pages	5
Journal	Bioresource Technology
Volume	123
DOIs	https://doi.org/10.1016/j.biortech.2012.07.101
State	Published - Nov 2012
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the grants from the National Basic Research Program of China (No. 2011CB707403 ), the National Natural Science Foundation of China (Nos. 31030001, 30970026 and 31070036 ).

Keywords

Cellulase
Gene knockout
PDE01641
Penicillium decumbens
Strain engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Improved cellulase production via disruption of PDE01641 in cellulolytic fungus Penicillium decumbens",

abstract = "To investigate the function of PDE01641, the homologue of Neurospora crassa NCU05137, during cellulase production in Penicillium decumbens, the PDE01641 encoding gene was knocked out in the present work. The PDE01641 deletion mutant showed enhanced cellobiohydrolase activity and β-glucosidase activity compared to the parental strain 114-2. Increased transcription of the main cellulase and hemicellulase genes in Δ. PDE01641 gave evidence that PDE01641 might affect the process associated with the regulation of cellulolytic enzymes expression. Furthermore, the deletion of PDE01641 from the genome of hypercellulolytic industrial strain JU-A10-T resulted in 36% and 80% increase in cellulase activity and hemicellulase activity respectively. These results revealed that PDE01641 plays an important role in the regulation of cellulolytic enzyme production in P. decumbens, and the engineering strain constructed in this work could be potentially used in bioenergy production.",

keywords = "Cellulase, Gene knockout, PDE01641, Penicillium decumbens, Strain engineering",

author = "Yanmei Zhang and Jiwei Zhang and Peng Xiao and Tianhong Wang and Yinbo Qu",

note = "Funding Information: This work was supported by the grants from the National Basic Research Program of China (No. 2011CB707403 ), the National Natural Science Foundation of China (Nos. 31030001, 30970026 and 31070036 ). ",

year = "2012",

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doi = "10.1016/j.biortech.2012.07.101",

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AU - Zhang, Yanmei

AU - Zhang, Jiwei

AU - Xiao, Peng

AU - Wang, Tianhong

AU - Qu, Yinbo

N1 - Funding Information: This work was supported by the grants from the National Basic Research Program of China (No. 2011CB707403 ), the National Natural Science Foundation of China (Nos. 31030001, 30970026 and 31070036 ).

PY - 2012/11

Y1 - 2012/11

N2 - To investigate the function of PDE01641, the homologue of Neurospora crassa NCU05137, during cellulase production in Penicillium decumbens, the PDE01641 encoding gene was knocked out in the present work. The PDE01641 deletion mutant showed enhanced cellobiohydrolase activity and β-glucosidase activity compared to the parental strain 114-2. Increased transcription of the main cellulase and hemicellulase genes in Δ. PDE01641 gave evidence that PDE01641 might affect the process associated with the regulation of cellulolytic enzymes expression. Furthermore, the deletion of PDE01641 from the genome of hypercellulolytic industrial strain JU-A10-T resulted in 36% and 80% increase in cellulase activity and hemicellulase activity respectively. These results revealed that PDE01641 plays an important role in the regulation of cellulolytic enzyme production in P. decumbens, and the engineering strain constructed in this work could be potentially used in bioenergy production.

AB - To investigate the function of PDE01641, the homologue of Neurospora crassa NCU05137, during cellulase production in Penicillium decumbens, the PDE01641 encoding gene was knocked out in the present work. The PDE01641 deletion mutant showed enhanced cellobiohydrolase activity and β-glucosidase activity compared to the parental strain 114-2. Increased transcription of the main cellulase and hemicellulase genes in Δ. PDE01641 gave evidence that PDE01641 might affect the process associated with the regulation of cellulolytic enzymes expression. Furthermore, the deletion of PDE01641 from the genome of hypercellulolytic industrial strain JU-A10-T resulted in 36% and 80% increase in cellulase activity and hemicellulase activity respectively. These results revealed that PDE01641 plays an important role in the regulation of cellulolytic enzyme production in P. decumbens, and the engineering strain constructed in this work could be potentially used in bioenergy production.

KW - Cellulase

KW - Gene knockout

KW - PDE01641

KW - Penicillium decumbens

KW - Strain engineering

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Improved cellulase production via disruption of PDE01641 in cellulolytic fungus Penicillium decumbens

Abstract

Bibliographical note

Keywords

UN SDGs

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