Improved microscopic observation of mammalian cells on microcarriers by fluorescent staining

Thomas J. Nikolai; Madhusudan V. Peshwa; Stephane Goetghebeur; Wei Shou Hu

doi:10.1007/BF00365430

Improved microscopic observation of mammalian cells on microcarriers by fluorescent staining

Thomas J. Nikolai, Madhusudan V. Peshwa, Stephane Goetghebeur, Wei Shou Hu

Chemical Engineering and Materials Science

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21 Scopus citations

Abstract

Many microcarriers used for the cultivation of animal cells do not allow for convenient microscopic observation of cell morphology and viability due to their optical properties. Using fluorescent viable stain combining fluorescein diacetate and ethidium bromide, we observed the distribution, morphology and viability of cells on various microcarriers.

Original language	English (US)
Pages (from-to)	141-146
Number of pages	6
Journal	Cytotechnology
Volume	5
Issue number	2
DOIs	https://doi.org/10.1007/BF00365430
State	Published - Feb 1991

Keywords

ethidium bromide
fluorescence
microcarriers
viability

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title = "Improved microscopic observation of mammalian cells on microcarriers by fluorescent staining",

abstract = "Many microcarriers used for the cultivation of animal cells do not allow for convenient microscopic observation of cell morphology and viability due to their optical properties. Using fluorescent viable stain combining fluorescein diacetate and ethidium bromide, we observed the distribution, morphology and viability of cells on various microcarriers.",

keywords = "ethidium bromide, fluorescence, microcarriers, viability",

author = "Nikolai, {Thomas J.} and Peshwa, {Madhusudan V.} and Stephane Goetghebeur and Hu, {Wei Shou}",

year = "1991",

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AU - Nikolai, Thomas J.

AU - Peshwa, Madhusudan V.

AU - Goetghebeur, Stephane

AU - Hu, Wei Shou

PY - 1991/2

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KW - ethidium bromide

KW - fluorescence

KW - microcarriers

KW - viability

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Improved microscopic observation of mammalian cells on microcarriers by fluorescent staining

Abstract

Keywords

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