Improved microscopic observation of mammalian cells on microcarriers by fluorescent staining

Thomas J. Nikolai, Madhusudan V. Peshwa, Stephane Goetghebeur, Wei Shou Hu

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Many microcarriers used for the cultivation of animal cells do not allow for convenient microscopic observation of cell morphology and viability due to their optical properties. Using fluorescent viable stain combining fluorescein diacetate and ethidium bromide, we observed the distribution, morphology and viability of cells on various microcarriers.

Original languageEnglish (US)
Pages (from-to)141-146
Number of pages6
JournalCytotechnology
Volume5
Issue number2
DOIs
StatePublished - Feb 1 1991

Keywords

  • ethidium bromide
  • fluorescence
  • microcarriers
  • viability

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