TY - JOUR
T1 - In vitro anticancer activities of some triterpene glycosides from holothurians of cucumariidae, stichopodidae, psolidae, holothuriidae and synaptidae families
AU - Fedorov, Sergey N.
AU - Dyshlovoy, Sergey A.
AU - Kuzmich, Alexandra S.
AU - Shubina, Larisa K.
AU - Avilov, Sergey A.
AU - Silchenko, Alexandra S.
AU - Bode, Ann M.
AU - Dong, Zigang
AU - Stonik, Valentin A.
N1 - Funding Information:
Financial support was provided by the Grant No. 14-25-00037 from the RSF (Russian Science Foundation). The authors are grateful to Dr. T.I. Antokhina (Severtsov Institute of Ecology and Evolution of the RAS, Moscow, Russia) for species identification of the starfish.
PY - 2016/9
Y1 - 2016/9
N2 - Triterpene glycosides isolated from holothurians are natural products known to possess cytotoxic properties against cancer cells. However, their anticancer prophylactic activity has not been studied sufficiently. The anticancer prophylactic, cytotoxic, and pro-apoptotic properties of 18 triterpene glycosides, as well as their effects on the transcriptional activities of activator protein-1 (AP-1) and nuclear factor-?B (NF-?B), were examined using methods that included EGF-induced JB6 Cl41 P+ cell transformation in soft agar, flow cytometry, MTS assessment of cell viability, and a luciferase activity assay. The compounds inhibited EGF-induced neoplastic JB6 Cl41 P+ cell transformation in soft agar and caused apoptosis and necrosis of human HL-60 and THP-1 leukemia cells. AP-1 and NF-?B were involved in the cellular response to the treatment by the compounds. Conclusion: glycosides isolated from holothurians of Cucumariidae, Stichopodidae, Psolidae, Holothuriidae and Synaptidae families have potential for development as new antitumor agents and as instruments to study AP-1 and NF-?B.
AB - Triterpene glycosides isolated from holothurians are natural products known to possess cytotoxic properties against cancer cells. However, their anticancer prophylactic activity has not been studied sufficiently. The anticancer prophylactic, cytotoxic, and pro-apoptotic properties of 18 triterpene glycosides, as well as their effects on the transcriptional activities of activator protein-1 (AP-1) and nuclear factor-?B (NF-?B), were examined using methods that included EGF-induced JB6 Cl41 P+ cell transformation in soft agar, flow cytometry, MTS assessment of cell viability, and a luciferase activity assay. The compounds inhibited EGF-induced neoplastic JB6 Cl41 P+ cell transformation in soft agar and caused apoptosis and necrosis of human HL-60 and THP-1 leukemia cells. AP-1 and NF-?B were involved in the cellular response to the treatment by the compounds. Conclusion: glycosides isolated from holothurians of Cucumariidae, Stichopodidae, Psolidae, Holothuriidae and Synaptidae families have potential for development as new antitumor agents and as instruments to study AP-1 and NF-?B.
KW - Anticancer prophylactic
KW - Apoptosis
KW - Necrosis
KW - Nuclear factors
KW - Triterpene glycosides
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U2 - 10.1177/1934578x1601100911
DO - 10.1177/1934578x1601100911
M3 - Article
AN - SCOPUS:85019113218
SN - 1934-578X
VL - 11
SP - 1239
EP - 1242
JO - Natural Product Communications
JF - Natural Product Communications
IS - 9
ER -
