In Vitro Assessment of the Efficacy of Thermal Therapy in Human Renal Cell Carcinoma

Lance P. Walsh, J. Kyle Anderson, Meredith R. Baker, Bumsoo Han, Jer Tsong Hsieh, Yair Lotan, Jeffrey A. Cadeddu

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Objectives: To define the temperature-time points that result in cell death in a human renal cell carcinoma cell (RCC) line in vitro. Methods: Cellular viability and clonogenic cell survival were determined for human A498 RCC cells after thermal treatment. Various temperature (45°C to 70°C) and time (1 to 30 minutes) combinations were used. Cell viability was assessed by vital dye uptake and clonogenic cell survival. Mathematical Arrhenius modeling was performed to construct a graphic display of A498 cell thermal sensitivity. Results: Temperature-time points at which 99% or greater cell death occurred according to the vital dye assay were 55°C for 30 minutes, 60°C for 10 minutes, and 65°C for 8 minutes. Clonogenic survival studies confirmed that cells treated at these temperature-time points failed to grow even after 10 days. Conclusions: These in vitro results show that short exposure to temperatures higher than 70°C is lethal in the A498 RCC cell line. Lower temperatures in the 60°C range require more prolonged heating to cause cell death. Knowledge of these temperatures will be useful to better plan and monitor complex radiofrequency ablations.

Original languageEnglish (US)
Pages (from-to)380-384
Number of pages5
JournalUrology
Volume70
Issue number2
DOIs
StatePublished - Aug 2007

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