In-vitro carbofuran induced micronucleus formation in human blood lymphocytes

R. K. Sharma, D. K. Rai, B. Sharma

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

The farmers in general get exposed to different chemicals including pesticides. Many of these compounds are capable of inducing mutations in DNA and lead to several diseases including cancer. Carbofuran is a broad spectrum pesticide and frequently used in agricultural practices in India. In this study we intended to evaluate DNA damage infl icted by pesticide exposure in human blood lymphocytes under in vitro condition. The lymphocytes were exposed to varying concentrations of carbofuran (0-50μM) and analyzed by means of the micronucleus (MN) test. The results obtained showed signifi cant increase in MN frequency after exposure to 5, 10, 25 and 50μM of carbofuran as compared to the control group. The frequencies of MN were observed to be in concentration dependent manner. As we further increase the concentration of carbofuran, we observed signifi cant decrease in the mean percentage of binucleated cells (70-49%) and increase in the number of micronu-clei formed per 1000 binucleated cells. Simultaneously, we also observed reduction in Cytokinesis-Block Proliferation index (CBPI) with increase in the carbofuran concentrations. The results indicate that this pesticide may exhibit genotoxic effect at higher concentrations. This study emphasizes the need to reinforce the good practices campaigns in order to enlighten those who work with pesticides and also to make them aware about the importance of using protective measures.

Original languageEnglish (US)
Pages (from-to)128-133
Number of pages6
JournalCellular and Molecular Biology
Volume58
Issue number1
DOIs
StatePublished - Dec 1 2012

Keywords

  • Carbofuran
  • Cytokinesis block proliferation index (CBPI)
  • DNA damage
  • Human blood lymphocytes
  • Micronucleus assay

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