In vitro development of human Killer-Immunoglobulin Receptor-positive NK cells.

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The in vitro culture system outlined in this chapter allows for the delineation of events that occur during the development of CD34(+) hematopoietic precursor cells into mature KIR(+) human NK cells. This system can also be utilized to study the effects of gene overexpression or knockdown on the process of NK cell differentiation through retroviral transduction and long-term culture. The necessary soluble factors and contact-dependent conditions for in vitro human NK cell development have been worked out in our laboratory over the past 16 years.

Original languageEnglish (US)
Pages (from-to)15-26
Number of pages12
JournalMethods in molecular biology (Clifton, N.J.)
Volume612
StatePublished - 2010

Fingerprint

Dive into the research topics of 'In vitro development of human Killer-Immunoglobulin Receptor-positive NK cells.'. Together they form a unique fingerprint.

Cite this