In vitro stimulation of bovine peripheral blood lymphocytes: comparison of round and flat-bottom microtiter plates for detection of tuberculin hypersensitivity

E. D. Sloane, Charles C Muscoplat, J. M. Kaneene, D. J. Klausner, C. O. Thoen, D. W. Johnson

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Lymphocytes from Mycobacterium bovis-sensitized and normal cattle were cultured in round- and/or flat-bottom microtiter plates and stimulated with M. bovis purified protein derivative (PPD) tuberculin. Blastogenic responses of lymphocytes from M. bovis-sensitized cattle to PPD cultured in round-bottom plates were significantly greater than those of lymphocytes cultured in flat-bottom microtiter plates. Normal lymphocytes of nonsensitized cattle were not stimulated by PPD in either round- or flat-bottom microtiter plates. Kinetics of lymphocyte responses in round-bottom plates are presented.

Original languageEnglish (US)
Pages (from-to)172-175
Number of pages4
JournalJournal of clinical microbiology
Volume7
Issue number2
StatePublished - 1978

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