Abstract
Lymphocytes from Mycobacterium bovis-sensitized and normal cattle were cultured in round- and/or flat-bottom microtiter plates and stimulated with M. bovis purified protein derivative (PPD) tuberculin. Blastogenic responses of lymphocytes from M. bovis-sensitized cattle to PPD cultured in round-bottom plates were significantly greater than those of lymphocytes cultured in flat-bottom microtiter plates. Normal lymphocytes of nonsensitized cattle were not stimulated by PPD in either round- or flat-bottom microtiter plates. Kinetics of lymphocyte responses in round-bottom plates are presented.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 172-175 |
| Number of pages | 4 |
| Journal | Journal of clinical microbiology |
| Volume | 7 |
| Issue number | 2 |
| State | Published - 1978 |